CD40 expression and function in murine B cell ontogeny

The CD40 antigen, a member of the nerve growth factor/tumor necrosis factor receptor family, is expressed on all mature B lymphocytes and plays a crucial role in B cell activation, T cell- dependent antigen-driven isotype switching and germinal center formation. We have analyzed C040 expression and...

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Bibliographic Details
Published in:International immunology 1996-03, Vol.8 (3), p.405-411
Main Authors: Castigli, Emanuela, Young, Faith, Carossino, Anna Maria, Alt, Frederick W., Geha, Raif S.
Format: Article
Language:English
Subjects:
Animals
B cell precursors
B cell transgenics
B-Lymphocytes - cytology
B-Lymphocytes - immunology
Base Sequence
Bone Marrow - immunology
CD40 Antigens - biosynthesis
CD40 Antigens - immunology
Cell Differentiation
Cell Division
Flow Cytometry
lymphoid development
Mice
Mice, Mutant Strains
Mice, Transgenic
Molecular Sequence Data
RAG-2-deficient mice
Receptors, IgE - biosynthesis
Spleen - immunology
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Summary:The CD40 antigen, a member of the nerve growth factor/tumor necrosis factor receptor family, is expressed on all mature B lymphocytes and plays a crucial role in B cell activation, T cell- dependent antigen-driven isotype switching and germinal center formation. We have analyzed C040 expression and function during mouse B cell development by examining B cell precursors in normal mice and in transgenic animals in which B cell development is frozen at discrete stages. These models included RAG-2 -/- mice, and transgenlc littermates that express μ heavy chain and/ or the bcl-2 proto-oncogene transgene. CD40 was undetectable at the pro-B cell stage, but was expressed, although at low levels, on pre-B cells. However, pre-B cells failed to respond to CD40 triggering either by expression of CD23 or by proliferation in the presence of lL-4. Overexpression of bcl-2 increased the density of CD40 expression on pre-B cells: these cells respond to CD40 ligation by expressing CD23 and by proliferating in the presence of IL-4.
ISSN:0953-8178
1460-2377
DOI:10.1093/intimm/8.3.405