Vitamin D receptor and growth inhibition by 1,25-dihydroxyvitamin D3 in human malignant melanoma cell lines

The expression of vitamin D receptors (VDR) and growth inhibition induced by 1,25-dihydroxyvitamin D3 have been noted in certain human malignant melanoma cell lines. In this study, widely disparate levels of VDR mRNA expression were demonstrated in a panel of eight human malignant melanoma cell line...

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Bibliographic Details
Published in:The Journal of surgical research 1996-02, Vol.61 (1), p.127-133
Main Authors: EVANS, S. R. T, HOUGHTON, A. M, SCHUMAKER, L, BRENNER, R. V, BURAS, R. R, FATEMEH DAVOODI, NAUTA, R. J, MOHSEN SHABAHANG
Format: Article
Language:English
Subjects:
Biological and medical sciences
Calcitriol - pharmacology
Cell Division - drug effects
Dermatology
Dose-Response Relationship, Drug
Humans
Medical sciences
Melanoma - metabolism
Melanoma - pathology
Receptors, Calcitriol - metabolism
Tumor Cells, Cultured
Tumors of the skin and soft tissue. Premalignant lesions
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Summary:The expression of vitamin D receptors (VDR) and growth inhibition induced by 1,25-dihydroxyvitamin D3 have been noted in certain human malignant melanoma cell lines. In this study, widely disparate levels of VDR mRNA expression were demonstrated in a panel of eight human malignant melanoma cell lines. Quantitation of receptor level by ligand binding assay showed a similar pattern. Proliferation and growth curve analysis was performed in two cell lines: RPMI 7951 (high VDR) and SK-MEL-28 (low VDR). Significant growth inhibition was noted in RPMI 7951 cells at 10(-9) M 1,25-dihydroxyvitamin D3. SK-MEL-28 cells, which express much lower levels of VDR, did not show any growth inhibition except at extremely high concentrations of 1,25-dihydroxyvitamin D3, namely 10(-5) M. These findings suggest a receptor-mediated mechanism of growth inhibition for 1,25-dihydroxyvitamin D3 and a role for this hormone in the growth of malignant melanoma cells.
ISSN:0022-4804
1095-8673
DOI:10.1006/jsre.1996.0092