Identification of matrix metalloproteinases and their inhibitor in the articular disc of the craniomandibular joint of the rabbit

Connective tissue cells synthesize and secrete matrix metalloproteinases (MMPs), a family of matrix-degrading enzymes (comprising collagenases, gelatinases and stromelysins), which are capable of degrading all the constituent molecules of connective tissues at physiological pH. This investigation do...

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Bibliographic Details
Published in:Archives of oral biology 1996-04, Vol.41 (4), p.315-322
Main Authors: Breckon, J.J.W., Hembry, R.M., Reynolds, J.J., Meikle, M.C.
Format: Article
Language:English
Subjects:
Animals
articular disc
Cartilage, Articular - enzymology
Cartilage, Articular - growth & development
Collagenases - analysis
Collagenases - biosynthesis
Dentistry
Extracellular Matrix - enzymology
fibrocartilage
Fluorescent Antibody Technique, Indirect
Gelatinases - analysis
Gelatinases - biosynthesis
Glycoproteins - analysis
Glycoproteins - metabolism
Matrix Metalloproteinase 3
matrix metalloproteinases
Metalloendopeptidases - analysis
Metalloendopeptidases - antagonists & inhibitors
Metalloendopeptidases - biosynthesis
Microscopy, Fluorescence
Protease Inhibitors - analysis
Protease Inhibitors - metabolism
Rabbits
temporomandibular joint
Temporomandibular Joint - enzymology
Temporomandibular Joint - growth & development
TIMP
Tissue Inhibitor of Metalloproteinases
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Summary:Connective tissue cells synthesize and secrete matrix metalloproteinases (MMPs), a family of matrix-degrading enzymes (comprising collagenases, gelatinases and stromelysins), which are capable of degrading all the constituent molecules of connective tissues at physiological pH. This investigation documents the synthesis and distribution of MMPs and their inhibitor TIMP-1 (tissue inhibitor of metalloproteinases-1) in the developing articular disc of the craniomandibular joint of the rabbit using indirect immunofluorescence microscopy. Cells of the disc synthesized all three classes of MMPs as well as TIMP-1 in all regions of the disc at all stages examined. MMPs and TIMP-1 were detected as bright intracellular accumulations probably within Golgi vesicles and as occasional diffuse, matrix-bound deposits. These results suggest that MMP-mediated matrix remodelling is a prominent feature of growth in craniomandibular joint disc.
ISSN:0003-9969
1879-1506
DOI:10.1016/0003-9969(95)00141-7