Targeted disruption of the murine tissue factor gene results in embryonic lethality

Tissue factor (TF) is an integral membrane glycoprotein that is believed to be the physiologic initiator of the blood coagulation cascade. Disruption of the mouse tissue factor gene leads to embryonic lethality between days E9.5-E11.5 of gestation. On E9.5, TF(-/-) embryos appear indistinguishable f...

Full description

Saved in:
Bibliographic Details
Published in:Blood 1996-09, Vol.88 (5), p.1583-1587
Main Authors: TOOMEY, J. R, KRATZER, K. E, LASKY, N. M, STANTON, J. J, BROZE, G. J
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood Coagulation - genetics
Blood coagulation. Blood cells
Chimera
Embryo, Mammalian - blood supply
Female
Fetal Death - genetics
Fetal Growth Retardation - genetics
Fundamental and applied biological sciences. Psychology
Gene Targeting
General aspects, investigation methods, hemostasis, fibrinolysis
Genetic Vectors - genetics
Gestational Age
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Molecular and cellular biology
Thromboplastin - deficiency
Thromboplastin - genetics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tissue factor (TF) is an integral membrane glycoprotein that is believed to be the physiologic initiator of the blood coagulation cascade. Disruption of the mouse tissue factor gene leads to embryonic lethality between days E9.5-E11.5 of gestation. On E9.5, TF(-/-) embryos appear indistinguishable from their TF(+/+) and TF(+/-) littermates. By E10.5, TF(-/-) embryos are severely growth retarded, appear nearly bloodless, and are in most cases dead. Initial observations suggest that TF(-/-) embryos are dying of circulatory failure. Approximately 15% of the TF(-/-) embryos survive beyond E10.5, but none complete gestation. Heterozygotes appear normal and free of bleeding complications.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.v88.5.1583.1583