Fluorescence polarization analysis of protein-DNA and protein-protein interactions

Fluorescence polarization is a powerful technique for characterizing macromolecular associations and can provide equilibrium determinations of protein-DNA and protein-protein interactions. This technique is particularly useful (and better suited than electrophoretic methods) to study low affinity pr...

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Bibliographic Details
Published in:Molecular endocrinology (Baltimore, Md.) Md.), 1996-06, Vol.10 (6), p.607-612
Main Authors: Lundblad, J R, Laurance, M, Goodman, R H
Format: Article
Language:English
Subjects:
Carrier Proteins - chemistry
Carrier Proteins - metabolism
Cyclic AMP Response Element-Binding Protein - chemistry
Cyclic AMP Response Element-Binding Protein - metabolism
DNA - chemistry
DNA - metabolism
Fluorescence Polarization - instrumentation
Fluorescence Polarization - methods
Models, Chemical
Models, Molecular
Proteins - chemistry
Proteins - metabolism
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Summary:Fluorescence polarization is a powerful technique for characterizing macromolecular associations and can provide equilibrium determinations of protein-DNA and protein-protein interactions. This technique is particularly useful (and better suited than electrophoretic methods) to study low affinity protein-protein interactions. In this review, we have outlined the principles underlying the use of fluorescence polarization to study the assembly of higher order complexes that bind to the CRE. The availability of simple, relatively inexpensive instrumentation means that this technology is no longer only within the realm of the physical biochemist.
ISSN:0888-8809
DOI:10.1210/me.10.6.607