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Single-cell measurements of quantal secretion induced by alpha-latrotoxin from rat adrenal chromaffin cells: dependence on extracellular Ca2
alpha-Latrotoxin (alpha-LT), from black widow spider venom, is a potent enhancer of the spontaneous quantal release of neurotransmitter from a variety of nerve terminals and clonal neurosecretory cells. Using electrochemical amperometry and estimation of membrane impedance by phase detection, we pre...
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Published in: | Pflügers Archiv 1996-10, Vol.432 (6), p.1039-1046 |
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description | alpha-Latrotoxin (alpha-LT), from black widow spider venom, is a potent enhancer of the spontaneous quantal release of neurotransmitter from a variety of nerve terminals and clonal neurosecretory cells. Using electrochemical amperometry and estimation of membrane impedance by phase detection, we present evidence that alpha-LT induces exocytosis of catecholamines from rat adrenal chromaffin cells beginning as rapidly as 30 s after close application of the toxin. This release is largely dependent on adequate levels of extracellular Ca2+ ([Ca2+]o). Lowering [Ca2+]o from 2 mM to |
doi_str_mv | 10.1007/s004240050232 |
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Using electrochemical amperometry and estimation of membrane impedance by phase detection, we present evidence that alpha-LT induces exocytosis of catecholamines from rat adrenal chromaffin cells beginning as rapidly as 30 s after close application of the toxin. This release is largely dependent on adequate levels of extracellular Ca2+ ([Ca2+]o). Lowering [Ca2+]o from 2 mM to </= 10-20 microM reduces the alpha-LT-induced rise in membrane capacitance by at least sixfold, on average, and nearly abolishes alpha-LT-induced quantal amperometric events, while still permitting insertion of non-selective cation channels. 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subjects | Adrenal Medulla - metabolism Animals Calcium - metabolism Calcium - pharmacology Catecholamines - metabolism Cell Membrane - physiology Cell Membrane Permeability Chromaffin System - metabolism Cytosol - metabolism Electric Conductivity Electrochemistry Exocytosis Ion Channels - metabolism Rats Spider Venoms - pharmacology |
title | Single-cell measurements of quantal secretion induced by alpha-latrotoxin from rat adrenal chromaffin cells: dependence on extracellular Ca2 |
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