Atomic Absorption Spectrophotometric Microdetermination of Total Mercury in Undigested Biological Samples

The influence of reaction time upon stannous (11) chloride as a reductant in the cold-vapor atomic absorption spectrophotometric method was investigated, in this comparative study, virtually all operating conditions, including volumes and chemicals used, were made identical except for the redesignin...

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Bibliographic Details
Published in:Journal of analytical toxicology 1988-05, Vol.12 (3), p.132-135
Main Authors: Ngim, Chun-Han, Foo, Swee-Cheng, Phoon, Wai-On
Format: Article
Language:English
Subjects:
Biological and medical sciences
Body Fluids - analysis
Chemical and industrial products toxicology. Toxic occupational diseases
Hair - analysis
Humans
Medical sciences
Mercury - analysis
Metals and various inorganic compounds
Spectrophotometry, Atomic
Toxicology
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Summary:The influence of reaction time upon stannous (11) chloride as a reductant in the cold-vapor atomic absorption spectrophotometric method was investigated, in this comparative study, virtually all operating conditions, including volumes and chemicals used, were made identical except for the redesigning of the reaction vessels. The use of s reaction mixing time of 2 minutes (TR method), rather than immediate bubbling with aspiration (IL method) of total mercury from undigested biological samples, has several advantages. There is an average 4.5-fold increase in sensitivity of peak height readings with virtually no loss of mercury vapor from the reaction vessel by diffusion. Using the TR method also eliminated interaction of mercury with the matrices investigated, viz., undigested blood, undigested saliva, undigested urine, and digested hair samples. The average recovery for the TR method was superior to the IL method, 99.3+3.2% vs. 84.7+3.3%, p
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/12.3.132