Nucleotide sequence analysis of cDNAs encoding a bovine galanin precursor protein in the adrenal medulla and chemical isolation of bovine gut galanin

A description is given of the primary structure of a bovine adrenal medullary precursor protein (123 amino acids), containing a nonrepetitive galanin sequence, different in four amino acid positions from pig galanin, structural information deduced from the nucleotide sequence analysis of two cDNA cl...

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Bibliographic Details
Published in:FEBS letters 1988-07, Vol.234 (2), p.400-406
Main Authors: Rökaeus, Åke, Carlquist, Mats
Format: Article
Language:English
Subjects:
Adrenal Medulla - metabolism
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Cattle
Chromaffin cell
DNA - genetics
DNA Restriction Enzymes
Fundamental and applied biological sciences. Psychology
Galanin
Galanin message-associated peptide
Genes. Genome
Hybridization histochemistry
Intestinal Mucosa - metabolism
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Neuropeptides - genetics
Peptide Fragments - analysis
Peptide isolation
Peptides - genetics
Peptides - isolation & purification
Protein Precursors - genetics
RNA hybridization
RNA, Messenger - genetics
Trypsin
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Summary:A description is given of the primary structure of a bovine adrenal medullary precursor protein (123 amino acids), containing a nonrepetitive galanin sequence, different in four amino acid positions from pig galanin, structural information deduced from the nucleotide sequence analysis of two cDNA clones. Pairs of lysine and arginine residues separate the galanin sequence N-terminally from the initiating methionine and a 29 amino acid leader peptide and C-terminally from a 59 amino acid peptide. The deduced amino acid composition was confirmed by the isolation and determination of the amino acid composition for bovine intestinal galanin. Northern blot analysis revealed the presence of an apparent single galanin mRNA species, of approx. 850–900 bases in size, which was present in a population of chromaffin cells scattered throughout the bovine adrenal medulla.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(88)80125-X