Increased membrane heterogeneity in stimulated human granulocytes

TMA-DPH fluorescence decay in human PMN before and after stimulation with FMLP was studied using frequency domain fluorometry. Membrane heterogeneity was assessed by the width of the continuous distributions of lifetime values of Lorentzian shape used to describe the fluorescence decay. In non-stimu...

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Bibliographic Details
Published in:FEBS letters 1988-07, Vol.234 (2), p.451-454
Main Authors: Valentino, Matteo, Governa, Mario, Gratton, Enrico, Fiorini, Rosamaria, Curatola, Giovanna, Bertoli, Enrico
Format: Article
Language:English
Subjects:
1-(4-Trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene
Biological and medical sciences
Cell Membrane - ultrastructure
Cell membranes. Ionic channels. Membrane pores
Cell structures and functions
Chemotaxis, Leukocyte
Fluorescence lifetime
FMLP, N-formyl-methionyl-leucyl-phenylalanine
Fundamental and applied biological sciences. Psychology
Granulocyte
HBSS, Hanks' balanced salt solution without red phenol
Humans
In Vitro Techniques
Kinetics
Membrane heterogeneity
Microdomain
Molecular and cellular biology
N-Formylmethionine Leucyl-Phenylalanine - pharmacology
Neutrophils - drug effects
Neutrophils - physiology
Neutrophils - ultrastructure
PMN, polymorphonuclear leukocyte
Spectrometry, Fluorescence
TMA-DPH, 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene
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Summary:TMA-DPH fluorescence decay in human PMN before and after stimulation with FMLP was studied using frequency domain fluorometry. Membrane heterogeneity was assessed by the width of the continuous distributions of lifetime values of Lorentzian shape used to describe the fluorescence decay. In non-stimulated granulocytes TMA-DPH fluorescence decay is characterized by two distributions of lifetime values centered at 6.5 and 1.0 ns and full width at half maximum of 0.3 and 1.2 ns, respectively. Within 15 min after stimulation, the center values of the two distribution components were 5.1 and 0.8 ns and the distribution width was 0.8 and 0.6 ns, respectively. These results indicate changes of membrane domain organization which can be ascribed to compositional changes redistribution of membrane components.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(88)80136-4