Use of DNA Probes and HEp-2 Cell Adherence Assay to Detect Diarrheagenic Escherichia coli

Four major categories of Escherichia coli are recognized as causes of diarrheal disease, including enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), and enterohemorrhagic E. coli (EHEC). The study described here was carried out in young children with acu...

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Bibliographic Details
Published in:The Journal of infectious diseases 1988-07, Vol.158 (1), p.224-228
Main Authors: Levine, Myron M., Prado, Valeria, Robins-Browne, Roy, Lior, Hermy, Kaper, James B., Moseley, Steve L., Gicquelais, Karen, Nataro, James P., Vial, Pablo, Tall, Ben
Format: Article
Language:English
Subjects:
Bacterial Adhesion
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Biological and medical sciences
Cell adhesion
Child, Preschool
Concise Clinical and Laboratory Communications
Diarrhea
Diarrhea - microbiology
Diarrhea, Infantile - microbiology
DNA probes
DNA, Bacterial
DNA, Recombinant
Enterotoxins
Escherichia coli
Escherichia coli - classification
Escherichia coli - isolation & purification
Escherichia coli - pathogenicity
Escherichia coli Infections - microbiology
Human bacterial diseases
Humans
Infant
Infectious diseases
Medical research
Medical sciences
Microbiology
Plasmids
Tumor Cells, Cultured
Volunteerism
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Summary:Four major categories of Escherichia coli are recognized as causes of diarrheal disease, including enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), and enterohemorrhagic E. coli (EHEC). The study described here was carried out in young children with acute diarrheal disease in Santiago, Chile, to assess the practicality of the EAF probe in identifying EPEC in coprocultures and to compare it with results of the HEp-2 cell assay and with definitive O serogrouping and to evaluate the sensitivity and specificity of a DNA probe to identify E. coli manifesting the true diffuse pattern of adherence in the HEp-2 assay.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/158.1.224