Comparison of procedures to detect Chlamydia psittaci antibodies in cockatiels (Nymphicus hollandicus)

Diagnostic serologic procedures for psittacine chlamydiosis were evaluated using experimentally inoculated cockatiels (Nymphicus hollandicus). Chlamydia psittaci was recovered from subjects on day 16 postinoculation. Seroconversion was confirmed by the direct complement fixation (DCF) test. The bloc...

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Bibliographic Details
Published in:Avian diseases 1996-04, Vol.40 (2), p.266-271
Main Authors: Tully, Thomas N., Shane, Simon M., Grimes, James E., Poston, Robert P., Kearney, Michael T.
Format: Article
Language:English
Subjects:
agglutination tests
Analysis of Variance
Animals
Antibodies
Antibodies, Bacterial - blood
anticorps
anticuerpos
Chick Embryo
Chlamydia psittaci
Chlamydophila psittaci
Chlamydophila psittaci - immunology
Chlamydophila psittaci - isolation & purification
Complement Fixation Tests
diagnosis
diagnostic
diagnostico
Eggs
elisa
Embryos
Enzyme-Linked Immunosorbent Assay - methods
Epidemiology
Feces
Feces - microbiology
Infections
Inoculation
Liver - pathology
Nymphicus hollandicus
pruebas de fijacion del complemento
Psittaciformes
Psittacosis - diagnosis
Psittacosis - immunology
Psittacosis - pathology
reacciones de aglutinacion
reaction d' agglutination
reaction de fixation du complement
Sensitivity and Specificity
Spleen - pathology
test elisa
Tissue culture techniques
Zoonoses
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Summary:Diagnostic serologic procedures for psittacine chlamydiosis were evaluated using experimentally inoculated cockatiels (Nymphicus hollandicus). Chlamydia psittaci was recovered from subjects on day 16 postinoculation. Seroconversion was confirmed by the direct complement fixation (DCF) test. The blocking enzyme-linked immunosorbent assay was demonstrated to be the most sensitive of three serodiagnostic procedures evaluated from inoculation until day 24 postinfection, with 92% sensitivity and 73% specificity. The DCF test was progressively more sensitive over the duration of the experiment, attaining 100% sensitivity and specificity on the day 24 postinfection. This study confirms the ability of the DCF procedure to adequately detect C. psittaci antibodies. Limitations relating to reproducibility of serologic results under clinical conditions found in this study indicate that a further refinement of tests is required to consistently detect and quantify antibodies.
ISSN:0005-2086
1938-4351
DOI:10.2307/1592219