Detection of genes coding for extended-spectrum SHV beta-lactamases in clinical isolates by a molecular genetic method, and comparison with the E test

A highly sensitive and specific method, termed PCR/NheI, for the detection of genes coding for SHV extended-spectrum beta-lactamases (ESBL) in clinical isolates is presented. It is based on polymerase chain reaction (PCR) amplification of the blaSHV genes, followed by restriction with NheI. Due to t...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 1996-05, Vol.15 (5), p.398-402
Main Authors: NÜESCH-INDERBINEN, M. T, HÄCHLER, H, KAYSER, F. H
Format: Article
Language:English
Subjects:
Base Sequence
beta-Lactamases - metabolism
Biological and medical sciences
Enterobacteriaceae
Escherichia coli - enzymology
Escherichia coli - genetics
General pharmacology
Genetic Techniques
Humans
Klebsiella pneumoniae - enzymology
Klebsiella pneumoniae - genetics
Medical sciences
Miscellaneous
Molecular Sequence Data
Pharmacology. Drug treatments
Polymerase Chain Reaction - methods
Sensitivity and Specificity
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Summary:A highly sensitive and specific method, termed PCR/NheI, for the detection of genes coding for SHV extended-spectrum beta-lactamases (ESBL) in clinical isolates is presented. It is based on polymerase chain reaction (PCR) amplification of the blaSHV genes, followed by restriction with NheI. Due to the glycine (positive 238) (SHV-non-ESBL)-->serine (position 238) (SHV-ESBL) mutation, only PCR fragments from the genes coding for SHV-ESBLs were cleaved. A commercially available test for ESBLs, the E test ESBL, identified 52% of our 29 clinical isolates carrying blaSHV-ESBL genes as ESBL producers.
ISSN:0934-9723
1435-4373
DOI:10.1007/BF01690097