Virus characterization and sequence of the fusion protein gene cleavage site of recent Newcastle disease virus field isolates from the southeastern United States and Puerto Rico

Nine Newcastle disease virus (NDV) isolates obtained from Puerto Rico, Georgia, Alabama, Mississippi, and Texas were analyzed for in vivo pathogenicity, biological properties (hemagglutination of mammalian erythrocytes), and for sequence variation at the amino acid and sense RNA level of the fusion...

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Bibliographic Details
Published in:Avian diseases 1996-04, Vol.40 (2), p.382-390
Main Authors: Marin, M.C. (University of Georgia, Athens, GA.), Villegas, P, Bennett, J.D, Seal, B.S
Format: Article
Language:English
Subjects:
ALABAMA (EU)
ALABAMA (EUA)
Amino Acid Sequence
Amino acids
Animals
Cattle
Chickens
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
Enzyme linked immunosorbent assay
EPREUVE D'HEMAGGLUTINATION
Erythrocytes
FILOGENIA
GEORGIA (EUA)
GEORGIE (EU)
Hemagglutination Tests
Horses
MISSISSIPPI
Molecular Sequence Data
Newcastle disease
Newcastle Disease - virology
Newcastle disease virus
Newcastle disease virus - classification
Newcastle disease virus - genetics
Newcastle disease virus - isolation & purification
Nucleotide sequences
Nucleotides
PATOGENICIDAD
PHYLOGENIE
Phylogeny
Polymerase Chain Reaction
PORTO RICO
Poultry
POUVOIR PATHOGENE
PRUEBAS DE HEMAGLUTINACION
PUERTO RICO
SECUENCIA NUCLEOTIDICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEOTIDIQUE
Sequencing
Southeastern United States
TEXAS
Vaccination
VARIACION GENETICA
VARIATION GENETIQUE
Viral Fusion Proteins - chemistry
Viral Fusion Proteins - genetics
Viral Vaccines
Virulence
VIRUS DE LA ENFERMEDAD DE NEWCASTLE
VIRUS MALADIE DE NEWCASTLE
Viruses
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Description
Summary:Nine Newcastle disease virus (NDV) isolates obtained from Puerto Rico, Georgia, Alabama, Mississippi, and Texas were analyzed for in vivo pathogenicity, biological properties (hemagglutination of mammalian erythrocytes), and for sequence variation at the amino acid and sense RNA level of the fusion protein cleavage site. Intracerebral pathogenicity index values ranged from 0 to 0.3 and the intravenous pathogenicity index obtained for all isolates was 0. Four isolates hemagglutinated bovine erythrocytes, whereas no hemagglutination was observed using equine erythrocytes. By direct sequencing of reverse transcription polymerase chain reaction products, all the isolates had a predicted fusion cleavage sequence comparable to lentogenic NDV strains. Based on nucleotide sequence, the viruses could be grouped phylogenetically with the B1 vaccine-type virus. However, nucleotide sequences were not 100% similar to the B1 or La Sota NDV strains, indicating that minor genetic heterogeneity occurs among lentogenic field isolates of NDV
ISSN:0005-2086
1938-4351
DOI:10.2307/1592236