Analysis of Cocaine, Benzoylecgonine, and Cocaethylene in Urine by HPLC with Diode Array Detection

A solid phase extraction method was developed for the isolation of cocaine, benzoylecgonine, and cocaethylene from urine followed by high-performance liquid chromatography/diode array detection. The application of a new solid hybrid phase extraction technology produced much cleaner extracts than con...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 1996-09, Vol.68 (17), p.3021-3028
Main Authors: Clauwaert, Karine M, Van Bocxlaer, Jan F, Lambert, Willy E, De Leenheer, André P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemistry
Chromatography, High Pressure Liquid
Cocaine
Cocaine - analogs & derivatives
Cocaine - urine
Drug addictions
Humans
Indicators and Reagents
Medical sciences
Narcotics - urine
Spectrophotometry, Ultraviolet
Substance Abuse Detection - methods
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A solid phase extraction method was developed for the isolation of cocaine, benzoylecgonine, and cocaethylene from urine followed by high-performance liquid chromatography/diode array detection. The application of a new solid hybrid phase extraction technology produced much cleaner extracts than conventional extraction procedures and made the selective extraction of substances with different polarities possible. Two internal standards with great structural resemblance to benzoylecgonine (a carboxylic acid) and to the two esters, cocaine and cocaethylene, respectively, were synthesized. A linear response over a broad concentration range was obtained. The sensitivity, specificity, and accuracy were satisfactory for each analyte. Hydrolysis of cocaine and cocaethylene to benzoylecgonine during extraction and analysis was less than 0.5%. The method described can be used to corroborate cocaine use, to establish cocaine overdoses, and to study pharmacological effects of cocaine and its metabolites.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac960060g