Suppression in the secretion of follicle-stimulating hormone and luteinizing hormone, and ovarian follicle development in heifers continuously infused with a gonadotropin-releasing hormone agonist

• Published in: Biology of reproduction 1996-07, Vol.55 (1), p.68-74
• Main Authors: Gong, J.G. (Roslin Institute, Edinburgh, Roslin, Midlothian, UK.), Campbell, B.K, Bramley, T.A, Gutierrez, C.G, Peters, A.R, Webb, R
• Format: Article
• Language: English
• Subjects: AGONISTAS; AGONISTE; Animals; Biological and medical sciences; Buserelin - administration & dosage; Buserelin - pharmacology; Cattle; CICLO ESTRAL; CYCLE OESTRAL; Drug Implants; Female; Follicle Stimulating Hormone - metabolism; FSH; Fundamental and applied biological sciences. Psychology; GENISSE; GONADOLIBERINE; HFS; HORMONA LIBERADORA DE GONADOTROPINA; HORMONAS; HORMONE; Hormone metabolism and regulation; INHIBICION; INHIBITION; Luteinizing Hormone - metabolism; Mammalian female genital system; NOVILLA; OVAIRE; Ovarian Follicle - drug effects; Ovarian Follicle - growth & development; OVARIOS; OVULACION; OVULATION; Ovulation - drug effects; PROGESTERONA; PROGESTERONE; Progesterone - blood; SECRECION; SECRETION; SERUM SANGUIN; SUERO SANGUINEO; Vertebrates: reproduction
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod55.1.68

A novel experimental model was developed in cattle to investigate the requirement for FSH and LH during ovarian follicle growth and development. On Day 5 of the estrous cycle, 7 heifers were each implanted with an osmotic minipump containing a GnRH agonist (GnRHa), Buserelin (release rate, 2.5 microgram/h). Another 7 heifers served as controls. Each minipump was replaced 28 days later with a second pump, which was left in place for a further 20 days. Blood samples were collected daily throughout the experimental period, and frequent samples were also collected on both days of minipump insertion and at 10 days after insertion of the second pump. The ovaries of all heifers were scanned daily by real-time ultrasonography to monitor follicular dynamics. All controls displayed 2 or 3 waves of FSH and follicular development per estrous cycle during the experiment. Insertion of the first minipump produced a large LH and FSH surge and induced ovulation in all 7 animals. Within 8 days of the start of treatment, serum LH concentrations fell to basal levels; they then remained constant at this level throughout the infusion period, only beginning to recover 4-5 days after the termination of infusion. After the initial increase, FSH returned to basal levels before showing a normal wave that was coincident with the emergence, growth, and regression of a dominant follicle. However, despite the peak levels of FSH, dominant follicles from the next wave failed to grow beyond 7-9 mm; they remained at this size for 3 wk until 3-4 days after insertion of the second minipump, when FSH fell precipitously to reach low levels that were maintained throughout the remainder of the infusion. After this fall in FSH concentrations, these follicles regressed rapidly, and no antral follicles 4 mm were detected until after the termination of treatment. Thereafter, FSH concentrations increased significantly; the increase was accompanied by the emergence of a follicular wave and development of a dom