Plasminogen activators, kallikrein-like proteinase and type I and type IV collagenases at various stages of oncogenic transformation

Comparative studies of membrane-associated, intracellular and secreted activities of serine (uPA, kallikrein-like proteinase) and metalloproteinases (type I and type IV collagenases) were carried out on rat embryo fibroblasts, sequentially immortalized and transformed by two different genes. Using t...

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Bibliographic Details
Published in:Immunopharmacology 1996-05, Vol.32 (1-3), p.131-134
Main Authors: Solovyeva, Nina I, Balayevskaya, Tatyana O, Dilakyan, Elvira A, Topol, Lilia Z, Kisseliov, Fedor L
Format: Article
Language:English
Subjects:
Animals
Cell Line, Transformed
Cell Transformation, Neoplastic - metabolism
Cell Transformation, Viral
Collagenase
Collagenases - metabolism
Cysteine proteinase
Fibroblast
Fibroblasts - enzymology
Kallikrein
Kallikreins - metabolism
Matrix Metalloproteinase 9
Oncogenic transformation
Plasminogen activator
Plasminogen Activators - metabolism
Rats
Rats, Inbred F344
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Summary:Comparative studies of membrane-associated, intracellular and secreted activities of serine (uPA, kallikrein-like proteinase) and metalloproteinases (type I and type IV collagenases) were carried out on rat embryo fibroblasts, sequentially immortalized and transformed by two different genes. Using this experimental model it was shown that (l) activity of uPA was expressed at the stage of immortalization solely; (2) intracellular and secreted activity of type I and IV collagenases decreased during process of transformation, (3) kallikrein-like proteinase activity was not revealed either in the primary or in transformed cells, (4) Z-Phe-Arg-MCA hydrolysis was the result of the action of cysteine proteinases alone; the increase in this activity was correlated with the stages of oncogenic transformation of fibroblasts.
ISSN:0162-3109
DOI:10.1016/0162-3109(95)00074-7