Loss of MHC I transcription trans-activator in the bovine B-LCL, BL3.1

BL3.1, a variant derived from the BLV infected bovine B cell line, BL3, is distinguished by a loss of expression of MHC class I. All surface MHC I products were down-regulated in BL3.1 compared with BL3 correlating with a diminution in MHC I heavy chain transcription. Class I genes, including regula...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 1996-05, Vol.51 (1), p.39-54
Main Authors: Harms, Jerome S., Splitter, Gary A.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
BoLA
bovinae
Bovine
bovine leukosis
Cattle
Cell Line
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
expresion genica
expression des genes
gene expression
Gene Expression Regulation
Genes, MHC Class I - genetics
Genes, MHC Class I - immunology
Genes, myc - immunology
Genetic Complementation Test
Humans
leucose bovine
leucosis bovina
Leukemia Virus, Bovine - genetics
major histocompatibility complex
Major histocompatibility complex class I
Promoter Regions, Genetic - genetics
retroviridae
sistema mhc
systeme mhc
Temperature
Trans-Activators - genetics
Transcription regulation
Transfection
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Summary:BL3.1, a variant derived from the BLV infected bovine B cell line, BL3, is distinguished by a loss of expression of MHC class I. All surface MHC I products were down-regulated in BL3.1 compared with BL3 correlating with a diminution in MHC I heavy chain transcription. Class I genes, including regulatory elements, showed no aberrations. The variant, BL3.1, did not differ from the parent cell line in expression of Bovine Leukemia Virus (BLV) or oncogene, c- myc. Transient transfection experiments determined the defect was trans rather than cis, and was due to loss of a trans-activator rather than gain of a trans-suppressor as evidenced by transient heterokaryon fusions. Southwestern blot analysis indicated that two DNA binding proteins associated with the MHC class I promoter were missing in BL3.1 cells. The specific response elements for these proteins in BL3 did not appear to be within the enhancerA region, the major enhancer region of the MHC I promoter.
ISSN:0165-2427
1873-2534
DOI:10.1016/0165-2427(95)05503-7