Cloning of the human sodium lodide symporter

The iodide concentrating activity of the thyroid gland is essential to the production of thyroid hormone and also provides a mechanism for the treatment of thyroid cancer by radioiodine ablation. We report here the nucleotide and amino acid sequence of the human sodium iodide symporter (hNIS), which...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-09, Vol.226 (2), p.339-345
Main Authors: Smanik, P A, Liu, Q, Furminger, T L, Ryu, K, Xing, S, Mazzaferri, E L, Jhiang, S M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Carrier Proteins - genetics
Cell Line
Cloning, Molecular
DNA, Complementary
Humans
Iodides - metabolism
Membrane Proteins - genetics
Molecular Sequence Data
Perchlorates - metabolism
Rats
Sequence Homology, Amino Acid
Symporters
Thyroid Neoplasms - genetics
Thyroid Neoplasms - pathology
Tumor Cells, Cultured
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Summary:The iodide concentrating activity of the thyroid gland is essential to the production of thyroid hormone and also provides a mechanism for the treatment of thyroid cancer by radioiodine ablation. We report here the nucleotide and amino acid sequence of the human sodium iodide symporter (hNIS), which mediates the iodide uptake activity in the thyroid gland. An open reading frame of 1929 nucleotides encodes a protein of 643 amino acids with 84% identity to the rat NIS (rNIS). Transient expression of the hNIS cDNA conferred perchlorate-sensitive iodide uptake to a nonthyroid cell line, COS-7. The expression of hNIS was detected at variable levels in papillary thyroid carcinoma tissues but not in any of the thyroid carcinoma cell lines that have lost the iodide uptake activity.
ISSN:0006-291X