Equal changes in L-type calcium channel density after 60 min of ischaemia in normal and ischaemically preconditioned porcine myocardium

Long‐lasting myocardial ischaemia reduces the density of sarcolemmal L‐type calcium channels (LCC). Ischaemic preconditioning protects the myocardium against development of infarction. The aim of this study was to investigate if ischaemia‐induced loss in LCC is affected by ischaemic preconditioning....

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Bibliographic Details
Published in:Acta physiologica Scandinavica 1996-06, Vol.157 (2), p.147-155
Main Authors: STOKKE, M., KIRKEBØEN, K. A., NÆSS, P. A., HAGELIN, E. M., ILEBEKK, A., BRØRS, O.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
calcium antagonists
Calcium Channel Blockers - pharmacokinetics
calcium channels
Calcium Channels - metabolism
Cardiology. Vascular system
Coronary heart disease
Female
Heart
Hemodynamics - physiology
In Vitro Techniques
ischaemic preconditioning
Ischemic Preconditioning, Myocardial
Isradipine - pharmacokinetics
Male
Medical sciences
Membranes - metabolism
myocardial ischaemia
Myocardial Ischemia - metabolism
Myocardium - metabolism
Swine
Ventricular Pressure - physiology
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Summary:Long‐lasting myocardial ischaemia reduces the density of sarcolemmal L‐type calcium channels (LCC). Ischaemic preconditioning protects the myocardium against development of infarction. The aim of this study was to investigate if ischaemia‐induced loss in LCC is affected by ischaemic preconditioning. Specific (+)‐[3H]isradipine binding to LCC was compared in membranes and homogenates from control and ischaemic regions of non‐preconditioned and ischaemically preconditioned hearts [two 10 min left anterior descending coronary artery (LAD) occlusions, each followed by 30 min reperfusion]. Biopsies were sampled after 60 min mid LAD occlusion from ischaemic and control (supplied by circumflex artery) regions. Sixty min ischaemia reduced binding density of specific (+)‐[3H]isradipine in membranes by 23±11% (n=7, P
ISSN:0001-6772
1365-201X
DOI:10.1046/j.1365-201X.1996.494229000.x