Detection of IgG and IgA Antibodies to Chlamydia trachomatis in Sera of Patients with Chlamydial Infections: Use of Immunoblotting and Immunoperoxidase Assays

• Published in: Sexually transmitted diseases 1988-04, Vol.15 (2), p.93-99
• Main Authors: HANUKA, NEGBA, GLASNER, MATTI, SAROV, ISRAEL
• Format: Article
• Language: English
• Subjects: Antibodies, Bacterial - analysis; Biological and medical sciences; Chlamydia Infections - immunology; Chlamydia trachomatis - immunology; Female; General aspects; Human infectious diseases. Experimental studies and models; Humans; Immunoassay; Immunoenzyme Techniques; Immunoglobulin A - analysis; Immunoglobulin G - analysis; Infectious diseases; Medical sciences; Original Articles
• ISSN: 0148-5717; 1537-4521
• DOI: 10.1097/00007435-198804000-00005

The immune response to individual structural polypeptides of Chlamydia trachomatis was studied in 75 sera from symptomatic and asymptomatic women with culture-proved genital infections and from apparently healthy women who were culture-negative for trachomatis. The immunoblotting technique and the single serovar (L₂) inclusion immunoperoxidase assays were used for measurement of the varous antibodies. Antibodies to 18 structural polypeptides, ranging in molecular weight from 29 to 204 Kdaltons, were detected by the immunoblotting technique in sera from seropositive women. The immunoperoxidase assay showed that sera with high titers of IgG and IgA antibodies to C. trachomatis reacted with more polypeptides than did sera with low titers in this test. Antibodies to the 60- and 62-Kdalton polypeptides were detected in almost all sera positive for IgG and IgA antibodies, irrespective of chiamydiai shedding. About 40% of sera with high IgG and IgA titers reacted with 39-, 57-, 64-, 72-, 86-, 105-, 155-, and 204-Kdalton polypeptides. The prevalence of IgA antibodies to C. trachomatis was higher among women with culture-proved chiamydiai infections than among apparently healthy controls.