A Comparison of an Enzyme Immunoassay and Cell Culture for Detection of Chlamydia trachomatis in Genito-Urinary Specimens

Chlamydia trachomatis infection is easy to treat but laborious to diagnose by culture. An antigen-detecting enzyme immunoassay (EIA; Chlamydiazyme®, Abbott Laboratories, North Chicago, IL), suitable for testing many samples, was compared with the conventional iodine-stained, one-passage culture. A t...

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Bibliographic Details
Published in:Sexually transmitted diseases 1988-04, Vol.15 (2), p.123-126
Main Authors: JENSEN, BIRGITTE LØKKE, HOFF, GERDI, WEISMANN, KAARE
Format: Article
Language:English
Subjects:
Bacteriological Techniques
Biological and medical sciences
Chlamydia Infections - diagnosis
Chlamydia Infections - microbiology
Chlamydia trachomatis - immunology
Chlamydia trachomatis - isolation & purification
Female
Fluorescent Antibody Technique
General aspects
Human infectious diseases. Experimental studies and models
Humans
Immunoenzyme Techniques
Infectious diseases
Male
Medical sciences
Original Articles
Sex Factors
Urethritis - diagnosis
Urethritis - microbiology
Uterine Cervicitis - diagnosis
Uterine Cervicitis - microbiology
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Summary:Chlamydia trachomatis infection is easy to treat but laborious to diagnose by culture. An antigen-detecting enzyme immunoassay (EIA; Chlamydiazyme®, Abbott Laboratories, North Chicago, IL), suitable for testing many samples, was compared with the conventional iodine-stained, one-passage culture. A total of 471 duplicate samples from 218 men (urethra) and 128 women (urethra and cervix) attending a sexually transmitted disease clinic were examined by both tests. The overall sensitivity, specificity, and predictive value of positive and negative results were 80.3%, 96.7%, 86.4%, and 95.0%, respectively. No difference between male and female patients was observed. A remarkable difference between sensitivities of female urethral (60.0%) and cervical (95.5%) samples was found. This difference is clearly important in cases of women with urethral infection only (19% in our study) and points to the need for further improvement of this EIA.
ISSN:0148-5717
1537-4521
DOI:10.1097/00007435-198804000-00013