FISH-Mapped CEPH YACs Spanning 0 to 46 cM on Human Chromosome 6

Seventy-six CEPH YACs were mapped by fluorescencein situhybridization (FISH) to human metaphase chromosomes. These clones have been ordered from pter to 46 cM by combining the results of FISH with sequence-tagged site content mapping using data from the public databases. This created a minimal tilin...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1996-08, Vol.36 (1), p.104-111
Main Authors: Bray-Ward, P., Bowlus, C., Choi, J., Paslier, D.Le, Weissenbach, J., Gruen, J.R.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromosome Mapping - methods
Chromosomes, Artificial, Yeast - genetics
Chromosomes, Human, Pair 6 - genetics
Classical genetics, quantitative genetics, hybrids
Databases, Factual
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Human
Humans
In Situ Hybridization, Fluorescence
Sequence Tagged Sites
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Summary:Seventy-six CEPH YACs were mapped by fluorescencein situhybridization (FISH) to human metaphase chromosomes. These clones have been ordered from pter to 46 cM by combining the results of FISH with sequence-tagged site content mapping using data from the public databases. This created a minimal tiling path containing at least 37 Mb of human genomic DNA from 0 to 46 cM on chromosome 6 that contains up to four gaps not greater than 200 kb. These data provide an integration of the FLpter physical map values with cytogenetic band localization and markers on the genetic and radiation hybrid maps. We also assessed YAC chimerism and placed three additional Whitehead contigs (WC952, WC799, WC436) within the integrated map.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1996.0430