Rubber Stoppers as Sources of Contaminants in Electrospray Analysis of Peptides and Proteins

Using MS/MS, we have identified ions at m/z 399, 421, 609.4, and 819.9 as those of tris(2-butoxyethyl) phosphate, originating in the gray rubber stoppers commonly used as closures in commercial sources of peptides and proteins. The compound is readily extracted by contact with organic solvents such...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1996-09, Vol.68 (18), p.3288-3289
Main Authors: Zhang, Xiaokui K, Dutky, Robert C, Fales, Henry M
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biochemistry
Biological and medical sciences
Contamination
Drug Packaging
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Ions
Mass Spectrometry
Peptides
Peptides - analysis
Proteins
Proteins - analysis
Rubber
Rubber - analysis
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Summary:Using MS/MS, we have identified ions at m/z 399, 421, 609.4, and 819.9 as those of tris(2-butoxyethyl) phosphate, originating in the gray rubber stoppers commonly used as closures in commercial sources of peptides and proteins. The compound is readily extracted by contact with organic solvents such as alcohol or acetic acid and even by water alone. Zinc salts of proteins are also observed, presumably from zinc oxide fillers present in the stoppers.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac960245n