The shape of human atrial action potential accounts for different frequency-related changes in vitro

• Published in: International journal of cardiology 1996-06, Vol.54 (3), p.237-249
• Main Authors: Dawodu, Amos Adeyemo, Monti, Francesco, Iwashiro, Katsunori, Schiariti, Michele, Chiavarelli, Roberta, Puddu, Paolo Emilio
• Format: Article
• Language: English
• Subjects: Action Potentials - physiology; Adult; Aged; Analysis of Variance; Atrial Function; Atrial trabeculae; Biological and medical sciences; Cardiac Surgical Procedures; Coronary Disease - diagnosis; Coronary Disease - physiopathology; Coronary Disease - surgery; Culture Techniques; Electrophysiology; Female; Frequency changes; Fundamental and applied biological sciences. Psychology; Heart; Heart Atria - anatomy & histology; Human action potential; Humans; In vitro electrophysiology; Male; Middle Aged; Time-dependent currents; Vertebrates: cardiovascular system; Voltage-dependent currents
• ISSN: 0167-5273; 1874-1754
• DOI: 10.1016/0167-5273(96)02605-8

We aimed at investigating frequency-related changes of human atrial action potential (AP) in vitro to see whether baseline AP shape might account for different responses to increasing stimulation rates. Human right atrial trabeculae ( n = 48) obtained from adult ( n = 38, mean age 59 ± 8, range 45–72 years) consecutive patients (≅30% of those operated upon by a single surgeon; 1.26 preparations per patient, range 1–2) were superfused in an organ bath with oxygenated (O 2 content 16 ml/l) and modified (NaHCO 3 25.7 mmol/l) Tyrode's solution at 31 °C. Baseline electrophysiology (pacing: 1 ms duration, 2–4 mA current intensity) at cycle length (CL) of 1000 ms was recorded in 90% (43 out of 48) of the preparations. The frequency-related protocol (CL from 1600 to 300 ms) was, however, undertaken in 23 (48%) preparations because 20 (42%) became pacing unresponsive immediately after baseline recordings. No statistical differences were seen when baseline electrophysiological parameters (mean ± SD) were grouped according to late pacing responsiveness ( n = 43 vs. n = 23): respectively, resting membrane potential (RMP) was −74 ± 6 vs. −75 ± 4 mV, maximal upstroke velocity (Vmax) 172 ± 60 vs. 173 ± 39 V/s, AP amplitude (APA) 89 ± 11 vs. 91 ± 8 mV and AP durations were at 30% (APD30%) 10 ± 13 vs. 13 ± 18 ms, 50% (APD50%) 45 ± 79 vs. 62 ± 91 ms and 90% (APD90%) 383 ± 103 vs. 407 ± 108 ms. To classify baseline AP shape, two criteria were adopted: criterion 1 (“objective”), based on APA (cut-off 90 mV) and APD90% (cut-off 500 ms) computed values and criterion 2 (“visual”) derived from the literature. These criteria enabled us to differentiate three AP shape types: type 1 (spike and dome), type 3 (no dome) and type 4 (extremely prolonged). At baseline, the two criteria diagnosed different proportions of AP shape types. There were, however, no intra-type statistical differences among electrophysiological parameters. By criterion 1, analysis of variance (ANOVA) showed significant inter-type differences of RMP, Vmax, APA, APD50 and 90% and by criterion 2 of APA, APD30, 50 and 90%, respectively. To facilitate comparisons with previous published data, criterion 2 was selected to analyse frequency-related changes of AP shape types. At low stimulation rate, ANOVA for repeated measures (with Greenhouse-Geisser \ ̂ g3 correction) showed inter-type differences for APD30, 50 and 90% ( P = 0.00005). RMP, Vmax, APA and APD90% were overall frequency-related ( P = 0.00005). Inter-type f