Effect of Acute Exhausting Exercise on Cytokine Gene Expression in Men

Because of recent interest in the effects of physical exercise on immunologic function, we decided to use state-of-the-art methods to evaluate cytokines in the peripheral blood leukocytes (PBLs) of 7 men before and after a maximal treadmill stress test. Change in cytokine gene expression was quantif...

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Bibliographic Details
Published in:International journal of sports medicine 1996-05, Vol.17 (4), p.299-302
Main Authors: Natelson, B. H., Zhou, X., Ottenweller, J. E., Bergen, M. T., Sisto, S. A., Drastal, S., Tapp, W. N., Gause, W. L.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Cytokines - biosynthesis
Cytokines - genetics
Exercise - physiology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gene Expression
Humans
Immunobiology
Immunology
Interleukins - biosynthesis
Interleukins - genetics
Leukocytes - immunology
Male
Modulation of the immune response (stimulation, suppression)
Polymerase Chain Reaction
Tumor Necrosis Factor-alpha - biosynthesis
Tumor Necrosis Factor-alpha - genetics
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Summary:Because of recent interest in the effects of physical exercise on immunologic function, we decided to use state-of-the-art methods to evaluate cytokines in the peripheral blood leukocytes (PBLs) of 7 men before and after a maximal treadmill stress test. Change in cytokine gene expression was quantified from PBLs using a reverse transcriptase polymerase chain reaction assay (RT-PCR). In contrast to reports on serum levels or using in vitro testing, direct gene expression of TNF-alpha decreased after the stress test (p < 0.008). However, the 47% decrease was relatively small and of questionable biological significance. Levels of IL-1a, IL-1b, IL-2, IL-4, IL-6, IL-10, and INF-gamma did not change.
ISSN:0172-4622
1439-3964
DOI:10.1055/s-2007-972850