Changes in calcium transport in mammalian sperm mitochondria and plasma membrane irradiated at 633 nm (HeNe laser)

The effect of light on calcium transport in mammalian sperm mitrochondria and plasma membrane was studied. Digitonine-treated spermatozoa and plasma membrane vesicles were irradiated with an HeNe laser at various powers and energy doses and Ca 2+ uptake was measured by the filtration method. It was...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 1996-07, Vol.34 (2), p.117-121
Main Authors: Breitbart, H., Levinshal, T., Cohen, N., Friedmann, H., Lubart, R.
Format: Article
Language:English
Subjects:
Animals
Biological Transport - radiation effects
Ca 2+ uptake
Calcium - metabolism
Cattle
Cell Membrane - metabolism
Cell Membrane - radiation effects
Cell Membrane Permeability
Digitonin - pharmacology
Digitonine treated sperm cells
HeNe laser
In Vitro Techniques
Lasers
Light
Male
Mitochondria - metabolism
Mitochondria - radiation effects
Plasma membrane vesicles
Spermatozoa - drug effects
Spermatozoa - metabolism
Spermatozoa - radiation effects
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Summary:The effect of light on calcium transport in mammalian sperm mitrochondria and plasma membrane was studied. Digitonine-treated spermatozoa and plasma membrane vesicles were irradiated with an HeNe laser at various powers and energy doses and Ca 2+ uptake was measured by the filtration method. It was found that there is an accelerate Ca 2+ uptake by the mitochondria after low power HeNe irradiation and inhibition after high power. The flux of Ca 2+ from the mitochondria was also examined and was found to be unaffected by the HeNe light. The ATP-dependent Ca 2+ uptake by the bovine plasma membrane vesicles was not changed by the HeNe irradiation.
ISSN:1011-1344
1873-2682
DOI:10.1016/1011-1344(95)07281-0