Adenosine A1 and A2 receptors modulate extracellular dopamine levels in rat striatum

• Published in: Neuroscience letters 1996-07, Vol.212 (1), p.53-56
• Main Authors: Okada, Motohiro, Mizuno, Kazuhisa, Kaneko, Sunao
• Format: Article
• Language: English
• Subjects: A1 receptor; A2a receptor; A2b receptor; Adenosine; Adenosine - analogs & derivatives; Adenosine - pharmacology; Animals; Antihypertensive Agents - pharmacology; Biological and medical sciences; Caffeine; Caffeine - pharmacology; Camptothecin - analogs & derivatives; Camptothecin - pharmacology; Central nervous system; Central neurotransmission. Neuromudulation. Pathways and receptors; Dopamine - metabolism; Dopamine release; Enzyme Inhibitors - pharmacology; Extracellular Space - metabolism; Fundamental and applied biological sciences. Psychology; Irinotecan; Male; Microdialysis; Neostriatum - chemistry; Neostriatum - metabolism; Phenethylamines - pharmacology; Phosphodiesterase Inhibitors - pharmacology; Purinergic P1 Receptor Agonists; Purinergic P1 Receptor Antagonists; Rats; Rats, Wistar; Receptors, Purinergic P1 - physiology; Striatum; Theobromine - analogs & derivatives; Theobromine - pharmacology; Vasodilator Agents - pharmacology; Vertebrates: nervous system and sense organs
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/0304-3940(96)12780-4

To clarify differences in the operating mechanisms of adenosine receptor subtypes (A1 and A2), striatal extracellular dopamine levels under various conditions were determined by in vivo microdialysis. Adenosine (50 μM) as well as the selective A1 agonist, 2-chloro- N 6-cyclopentyladenosine (CCPA; 1 μM) decreased striatal extracellular dopamine levels, while the selective adenosine A1 antagonist, 8-cyclopentyl-1,3-dimethylxantine (CPT; 50 μM) and caffeine (100 μM) increased striatal extracellular dopamine levels. A selective A2a agonist, 2-[4-(2-carboxyethyl)phenethylamino]-5′- N-ethylcarboxamideadenosine (CGS21680; 10 μM), a selective A2 agonist, N 6-[2-(3,5-dimethoxyphenyl)-2-(methylphenyl)ethyl]adenosine (DPMA; 5 μM) and a selective A2 antagonist, 3,7-dimethyl-1-propargylxanthine (DMPX; 10 μM), did not affect extracellular dopamine levels. When the A1 receptor was blocked by CPT, extracellular dopamine levels were increased by adenosine and DPMA, decreased by caffeine as well as DMPX, and unaffected by CGS21680. These results indicate that the stimulatory effects of the A2 receptor on striatal extracellular dopamine levels are masked by the inhibitory effects of the A1 receptor.