Determination of benflumetol in human plasma by reversed-phase high-performance liquid chromatography with ultraviolet detection

A reversed-phase high-performance liquid chromatographic method for the determination of benflumetol in human plasma is described. Benflumetol in plasma samples was extracted with a glacial acetic acid-ethyl acetate (1:100, v/v) mixture at pH 4.0. Chromatography was performed on a Spherisorb C 18 co...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Biomedical applications Biomedical applications, 1996-06, Vol.681 (2), p.299-306
Main Authors: Zeng, Mei-Yi, Lu, Zhi-Liang, Yang, Song-Cheng, Zhang, Min, Liao, Jie, Liu, Shu-Ling, Teng, Xi-He
Format: Article
Language:English
Subjects:
Acetic Acid
Adult
Antimalarials - blood
Benflumetol
Chromatography, High Pressure Liquid - methods
Chromatography, High Pressure Liquid - standards
Chromatography, High Pressure Liquid - statistics & numerical data
Diethylamines
Ethanolamines - blood
Fluorenes - blood
Humans
Lumefantrine
Methanol
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A reversed-phase high-performance liquid chromatographic method for the determination of benflumetol in human plasma is described. Benflumetol in plasma samples was extracted with a glacial acetic acid-ethyl acetate (1:100, v/v) mixture at pH 4.0. Chromatography was performed on a Spherisorb C 18 column using a methanol-water-glacial acetic acid-diethyl amine (93:6:1:0.03, v/v) mixture as the mobile phase and UV-VIS detection at 335 nm. The identity and purity of the benflumetol peak were carefully examined, and the internal standard method was applied for its quantitation. The absolute recovery of benflumetol in spiked plasma samples was 92.91% over the concentration range 5–4000 ng/ml. The recovery of internal standard “8212” at a concentration of 300 ng/ml in spiked plasma was 84.85%. The detection limit of benflumetol was 11.8 ng/ml. Plasma concentration-time profiles in healthy volunteer adults were measured after a single-dose oral administration of 500 mg of benflumetol. The assay procedures were within the quality control limits.
ISSN:0378-4347
1572-6495
DOI:10.1016/0378-4347(95)00542-0