Determination of 1-O-acyl-2-acetyl- sn-glyceryl-3-phosphorylcholine, platelet-activating factor and related phospholipids in biological samples by high-performance liquid chromatography-tandem mass spectrometry

Combining normal-phase HPLC separation and tandem mass spectrometric detection, using an ion-spray HPLC-MS interface, a quantitative method for acyl-platelet activating factor (acyl-PAF), platelet-activating factor (PAF) and related phospholipids was developed. Mass spectra, positive ions, showed in...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical applications Biomedical applications, 1996-06, Vol.682 (1), p.35-45
Main Authors: Rizea Savu, S., Silvestro, L., Sörgel, F., Montrucchio, G., Lupia, E., Camussi, G.
Format: Article
Language:English
Subjects:
1-O-Acyl-2-acetyl- sn-glyceryl-3-phosphorylcholine
Cells, Cultured
Chromatography, High Pressure Liquid
Endothelium, Vascular - chemistry
Endothelium, Vascular - cytology
Humans
Linear Models
Lysophosphatidylcholines - analysis
Lysophosphatidylcholines - chemistry
Mass Spectrometry
Phospholipid Ethers - analysis
Phospholipid Ethers - chemistry
Phospholipids
Phospholipids - analysis
Phospholipids - chemistry
Platelet Activating Factor - analogs & derivatives
Platelet Activating Factor - analysis
Platelet Activating Factor - chemistry
Platelet-activating factor
Reference Standards
Umbilical Veins
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Summary:Combining normal-phase HPLC separation and tandem mass spectrometric detection, using an ion-spray HPLC-MS interface, a quantitative method for acyl-platelet activating factor (acyl-PAF), platelet-activating factor (PAF) and related phospholipids was developed. Mass spectra, positive ions, showed intense [M+H] + ions; collision-induced dissociation of protonated molecular ions gave characteristic daughter ions corresponding to the polar head. Detection limits of 0.1–0.3 ng injected were obtained by multiple reaction monitoring. Samples of human endothelial cells treated with compounds modulating the levels of acyl-PAF and PAF have been analyzed by the present technique, proving that this approach is suitable for biochemical studies.
ISSN:0378-4347
1572-6495
DOI:10.1016/0378-4347(96)00070-9