Hormonal regulation of lipoprotein lipase activity from 5-day-old rat hepatocytes

Lipoprotein lipase (LPL) activity is known to be synthesized, active and functional in the 1-day-old rat liver: it peaks just at birth triggered by parturition. During suckling LPL mRNA, LPL synthesis and LPL activity are still high at 5 days and then fade reaching adult values at weaning. How LPL e...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 1996-01, Vol.116 (1), p.97-104
Main Authors: Julve, J., Robert, M.Q., Llobera, M., Peinado-Onsurbe, J.
Format: Article
Language:English
Subjects:
Animals
Animals, Suckling
Dexamethasone - administration & dosage
Dexamethasone - pharmacology
Dose-Response Relationship, Drug
Female
Glucagon - administration & dosage
Glucagon - pharmacology
Heparin - pharmacology
Hormones - pharmacology
In Vitro Techniques
Lipoprotein Lipase - metabolism
Liver
Liver - drug effects
Liver - enzymology
LPL
LPL-extinction
Male
Neonatal
Rat
Rats
Rats, Wistar
Suckling
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Summary:Lipoprotein lipase (LPL) activity is known to be synthesized, active and functional in the 1-day-old rat liver: it peaks just at birth triggered by parturition. During suckling LPL mRNA, LPL synthesis and LPL activity are still high at 5 days and then fade reaching adult values at weaning. How LPL expression is gradually extinguished is not known. Therefore we studied the effect of different doses of several hormones on LPL activity released by incubated hepatocytes from 5-day-old rats. In the presence of heparin the release of LPL activity in the medium was linear until 3 h and was always significantly increased vs. without heparin. At 3 h in the presence of heparin the main hormonal effects were: dose-independent increase (30–60%) with dexamethasone; dose-dependent increase (20–60%) with glucagon; dose-independent decrease (50–60%) with ethinylestradiol, testosterone, progesterone and prolactin; no effect with insulin; 20–40% increase with adrenaline
ISSN:0303-7207
1872-8057
DOI:10.1016/0303-7207(95)03704-7