Down-regulation of Na channel expression by A23187 in N1E-115 neuroblastoma cells

Growth of cultured N1E-115 neuroblastoma cells in 1 microM A23187 for 2 days to elevate internal Ca reduced both membrane Na current and the transient, but not steady state, component of outward K current. Na channel mRNA abundance was reduced by an average value of 45% without effect on Kv3.1. Incr...

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Bibliographic Details
Published in:Brain research 1996-01, Vol.706 (2), p.343-346
Main Authors: HIRSH, J. K, QUANDT, F. N
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Calcimycin - pharmacology
Cell membranes. Ionic channels. Membrane pores
Cell structures and functions
Down-Regulation
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Molecular Sequence Data
Neuroblastoma
Neurons - drug effects
Neurons - metabolism
Polymerase Chain Reaction
RNA, Messenger - biosynthesis
Sodium Channels - drug effects
Sodium Channels - genetics
Tumor Cells, Cultured
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Summary:Growth of cultured N1E-115 neuroblastoma cells in 1 microM A23187 for 2 days to elevate internal Ca reduced both membrane Na current and the transient, but not steady state, component of outward K current. Na channel mRNA abundance was reduced by an average value of 45% without effect on Kv3.1. Increases in internal Ca may therefore control excitability by independent regulation of Na and K channel mRNA abundance in neurons.
ISSN:0006-8993
1872-6240
DOI:10.1016/0006-8993(95)01340-7