Repetitive applications of ATP potentiate potassium current by Ca2+/calmodulin kinase in cultured rat hippocampal neurons

ATP evoked whole-cell potassium currents in hippocampal neurons. The second application of ATP to the same cell potentiated the current amplitude to around 140% and the current potentiation was maintained by further applications. A calmodulin inhibitor, W-7, or a selective Ca2+/calmodulin-dependent...

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Bibliographic Details
Published in:Neuroscience letters 1996-01, Vol.203 (2), p.115-118
Main Authors: IKEUCHI, Y, NISHIZAKI, T, OKADA, Y
Format: Article
Language:English
Subjects:
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine - analogs & derivatives
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine - pharmacology
Adenosine Triphosphate - administration & dosage
Adenosine Triphosphate - pharmacology
Animals
Animals, Newborn
Biological and medical sciences
Biological Transport - drug effects
Calcium - metabolism
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases - antagonists & inhibitors
Calcium-Calmodulin-Dependent Protein Kinases - metabolism
Cells, Cultured
Central nervous system
Electrophysiology
Enzyme Inhibitors - pharmacology
Fundamental and applied biological sciences. Psychology
Hippocampus - cytology
Indoles - pharmacology
Ion Channel Gating - drug effects
Isoquinolines - pharmacology
Maleimides - pharmacology
Neurons - drug effects
Neurons - physiology
Patch-Clamp Techniques
Potassium - metabolism
Protein Kinase C - antagonists & inhibitors
Protein Kinase C - metabolism
Rats
Signal Transduction - drug effects
Sulfonamides - pharmacology
Vertebrates: nervous system and sense organs
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Summary:ATP evoked whole-cell potassium currents in hippocampal neurons. The second application of ATP to the same cell potentiated the current amplitude to around 140% and the current potentiation was maintained by further applications. A calmodulin inhibitor, W-7, or a selective Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor, KN-62, inhibited the current potentiation, although a selective protein kinase C inhibitor, GF109203X, or a selective cAMP-dependent protein kinase inhibitor, H-89, had no effect. In addition, ATP enhanced intracellular free Ca2+ concentration, which may activate CaMKII, but this enhancement was blocked by repetitive applications. These results provide an indication that CaMKII may be involved in the current potentiation by repetitive applications of ATP.
ISSN:0304-3940
1872-7972
DOI:10.1016/0304-3940(95)12276-1