A new approach to studies of cell-antibody interactions using fluorescent lipid probes

The interaction of poly- and monoclonal antibodies against the L-chain of human Ig with Burkitt lymphoma EB-3 cells was studied using a fluorescent lipid probe, anthrylvinyl-labelled sphingomyelin, incorporated into the cell plasma membrane. Binding of the antibodies to Ig receptors on the surface w...

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Bibliographic Details
Published in:FEBS letters 1988-08, Vol.236 (2), p.315-317
Main Authors: Tonevitsky, A.G., Manevich, E.M., Zhukova, O.S., Arsen'eva, E.L., Bogacheva, G.T., Bergelson, L.D.
Format: Article
Language:English
Subjects:
Antibodies, Anti-Idiotypic
Antigen-Antibody Reactions
ASM, anthrylvinyl-labelled sphingomyelin, N-(12-(9-anthryl)-11-trans-dodecenoyl)sphingosyl-1-phosphocholine
Burkitt Lymphoma
Burkitt lymphoma cell
Cell Membrane - immunology
Fluorescence Polarization - methods
Fluorescent Dyes
Fluorescent probe
Humans
Ig, immunoglobin
Immunoglobulin Light Chains
Immunoglobulin receptor
Lipid fluorescence
Membrane Lipids
P, fluorescence polarization
Receptors, Antigen, B-Cell
Sphingomyelins
Tumor Cells, Cultured
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Summary:The interaction of poly- and monoclonal antibodies against the L-chain of human Ig with Burkitt lymphoma EB-3 cells was studied using a fluorescent lipid probe, anthrylvinyl-labelled sphingomyelin, incorporated into the cell plasma membrane. Binding of the antibodies to Ig receptors on the surface was shown to induce changes in the fluorescence polarization of the probe. The high sensitivity of the method allows one to detect less than 100 antibody molecules per cell. The possibility of using cells or liposomes carrying antigens and fluorescent lipids for the determination of antibodies in solution is discussed.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(88)80045-0