Molecular polymorphism and phenotypic variation in Aspergillus carbonarius

Thirteen collection strains and field isolates of Aspergillus carbonarius were examined by using various genotypic and phenotypic approaches. Restriction fragment length polymorphism analysis of the ribosomal RNA gene cluster and the mitochondrial DNA of the strains revealed only slight variations,...

Full description

Saved in:
Bibliographic Details
Published in:Antonie van Leeuwenhoek 1996-07, Vol.70 (1), p.59-66
Main Authors: KEVEI, F, HAMARI, Z, VARGA, J, KOZAKIEWICZ, Z, CROFT, J. H
Format: Article
Language:English
Subjects:
Acid Phosphatase - metabolism
Aspergillus - genetics
Aspergillus carbonarius
Biological and medical sciences
Blotting, Southern
Catalase - metabolism
DNA Primers
DNA, Bacterial
DNA, Mitochondrial - genetics
Esterases - metabolism
Fundamental and applied biological sciences. Psychology
Genotype
Microbiology
Mycology
Oxidoreductases - metabolism
Phenotype
Phylogeny
Polymerase Chain Reaction
Polymorphism, Genetic - genetics
Polymorphism, Restriction Fragment Length
RNA, Ribosomal
Superoxide Dismutase - metabolism
Systematics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Thirteen collection strains and field isolates of Aspergillus carbonarius were examined by using various genotypic and phenotypic approaches. Restriction fragment length polymorphism analysis of the ribosomal RNA gene cluster and the mitochondrial DNA of the strains revealed only slight variations, except for one field isolate (IN7), which exhibited completely different ribosomal RNA gene cluster and mitochondrial DNA patterns. The mitochondrial DNAs of these strains were found to be much larger (45 to 57 kb) than those found earlier in the A. niger aggregate. Strain-specific characters could be detected by the random amplified polymorphic DNA technique. Isoenzyme analysis and examination of carbon source utilisation patterns of the strains also revealed some intraspecific variability, though much smaller than that observed by using DNA-based techniques. The dendrograms constructed based on genotypic and phenotypic data suggest that strain IN7 might represent a new subspecies of A. carbonarius.
ISSN:0003-6072
1572-9699
DOI:10.1007/BF00393570