Monoamine oxidase gene transcription in human cell lines : treatment with psychoactive drugs and ethanol

In the present study transcriptional activities has been measured with different fragments of the 5'-flanking sequence of the human monoamine oxidase (MAO) genes linked to human growth hormone which was used as a reporter gene. SH-SY5Y neuroblastoma cells and 1242 MG glioma cells were compared...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Neural Transmission 1996-01, Vol.103 (6), p.681-692
Main Authors: EKBLOM, J, ZHU, Q.-S, CHEN, K, SHIH, J. C
Format: Article
Language:English
Subjects:
Alcohol Drinking
Antimanic Agents - pharmacology
Antipsychotic Agents - pharmacology
Astrocytoma
Biological and medical sciences
Central Nervous System Depressants - pharmacology
Dose-Response Relationship, Drug
Electrophoresis
Ethanol - pharmacology
Fundamental and applied biological sciences. Psychology
Humans
Isolated neuron and nerve. Neuroglia
Lithium Chloride - pharmacology
Monoamine Oxidase - genetics
Monoamine Oxidase Inhibitors - pharmacology
Neuroblastoma
Promoter Regions, Genetic - physiology
Psychotropic Drugs - pharmacology
Raclopride
Salicylamides - pharmacology
Selegiline - pharmacology
Transcription, Genetic - drug effects
Transcription, Genetic - physiology
Tumor Cells, Cultured - drug effects
Tumor Cells, Cultured - enzymology
Vertebrates: nervous system and sense organs
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present study transcriptional activities has been measured with different fragments of the 5'-flanking sequence of the human monoamine oxidase (MAO) genes linked to human growth hormone which was used as a reporter gene. SH-SY5Y neuroblastoma cells and 1242 MG glioma cells were compared under basal conditions as well as after treatments with different drugs. Under basal conditions, the relative reporter activities of the different promoter fragments were similar for both cell lines. No changes in promoter activities, were observed when cells were treated with L-deprenyl, lithium chloride or raclopride. In contrast, increases (2-3-fold) in both reporter gene expression and enzyme activity were observed after ethanol treatment of cells transfected with MAO-B fragments. Gel retardation analysis showed that ethanol caused changes in transcription factor binding to the MAO-B core promoter in both the SH-SY5Y and 1242 MG cell lines in a cell-type specific fashion.
ISSN:0300-9564
1435-1463
DOI:10.1007/BF01271228