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Monoamine oxidase gene transcription in human cell lines : treatment with psychoactive drugs and ethanol
In the present study transcriptional activities has been measured with different fragments of the 5'-flanking sequence of the human monoamine oxidase (MAO) genes linked to human growth hormone which was used as a reporter gene. SH-SY5Y neuroblastoma cells and 1242 MG glioma cells were compared...
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| Published in: | Journal of Neural Transmission 1996-01, Vol.103 (6), p.681-692 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c342t-f163e42e8f793fedabc244e16bc05c867efd30b4dd7eee5a058c32f46558a8263 |
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| cites | cdi_FETCH-LOGICAL-c342t-f163e42e8f793fedabc244e16bc05c867efd30b4dd7eee5a058c32f46558a8263 |
| container_end_page | 692 |
| container_issue | 6 |
| container_start_page | 681 |
| container_title | Journal of Neural Transmission |
| container_volume | 103 |
| creator | EKBLOM, J ZHU, Q.-S CHEN, K SHIH, J. C |
| description | In the present study transcriptional activities has been measured with different fragments of the 5'-flanking sequence of the human monoamine oxidase (MAO) genes linked to human growth hormone which was used as a reporter gene. SH-SY5Y neuroblastoma cells and 1242 MG glioma cells were compared under basal conditions as well as after treatments with different drugs. Under basal conditions, the relative reporter activities of the different promoter fragments were similar for both cell lines. No changes in promoter activities, were observed when cells were treated with L-deprenyl, lithium chloride or raclopride. In contrast, increases (2-3-fold) in both reporter gene expression and enzyme activity were observed after ethanol treatment of cells transfected with MAO-B fragments. Gel retardation analysis showed that ethanol caused changes in transcription factor binding to the MAO-B core promoter in both the SH-SY5Y and 1242 MG cell lines in a cell-type specific fashion. |
| doi_str_mv | 10.1007/BF01271228 |
| format | article |
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C</creator><creatorcontrib>EKBLOM, J ; ZHU, Q.-S ; CHEN, K ; SHIH, J. C</creatorcontrib><description>In the present study transcriptional activities has been measured with different fragments of the 5'-flanking sequence of the human monoamine oxidase (MAO) genes linked to human growth hormone which was used as a reporter gene. SH-SY5Y neuroblastoma cells and 1242 MG glioma cells were compared under basal conditions as well as after treatments with different drugs. Under basal conditions, the relative reporter activities of the different promoter fragments were similar for both cell lines. No changes in promoter activities, were observed when cells were treated with L-deprenyl, lithium chloride or raclopride. In contrast, increases (2-3-fold) in both reporter gene expression and enzyme activity were observed after ethanol treatment of cells transfected with MAO-B fragments. Gel retardation analysis showed that ethanol caused changes in transcription factor binding to the MAO-B core promoter in both the SH-SY5Y and 1242 MG cell lines in a cell-type specific fashion.</description><identifier>ISSN: 0300-9564</identifier><identifier>EISSN: 1435-1463</identifier><identifier>DOI: 10.1007/BF01271228</identifier><identifier>PMID: 8836930</identifier><identifier>CODEN: JNTMAH</identifier><language>eng</language><publisher>Wien: Springer</publisher><subject>Alcohol Drinking ; Antimanic Agents - pharmacology ; Antipsychotic Agents - pharmacology ; Astrocytoma ; Biological and medical sciences ; Central Nervous System Depressants - pharmacology ; Dose-Response Relationship, Drug ; Electrophoresis ; Ethanol - pharmacology ; Fundamental and applied biological sciences. Psychology ; Humans ; Isolated neuron and nerve. Neuroglia ; Lithium Chloride - pharmacology ; Monoamine Oxidase - genetics ; Monoamine Oxidase Inhibitors - pharmacology ; Neuroblastoma ; Promoter Regions, Genetic - physiology ; Psychotropic Drugs - pharmacology ; Raclopride ; Salicylamides - pharmacology ; Selegiline - pharmacology ; Transcription, Genetic - drug effects ; Transcription, Genetic - physiology ; Tumor Cells, Cultured - drug effects ; Tumor Cells, Cultured - enzymology ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of Neural Transmission, 1996-01, Vol.103 (6), p.681-692</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-f163e42e8f793fedabc244e16bc05c867efd30b4dd7eee5a058c32f46558a8263</citedby><cites>FETCH-LOGICAL-c342t-f163e42e8f793fedabc244e16bc05c867efd30b4dd7eee5a058c32f46558a8263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3165042$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8836930$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>EKBLOM, J</creatorcontrib><creatorcontrib>ZHU, Q.-S</creatorcontrib><creatorcontrib>CHEN, K</creatorcontrib><creatorcontrib>SHIH, J. C</creatorcontrib><title>Monoamine oxidase gene transcription in human cell lines : treatment with psychoactive drugs and ethanol</title><title>Journal of Neural Transmission</title><addtitle>J Neural Transm (Vienna)</addtitle><description>In the present study transcriptional activities has been measured with different fragments of the 5'-flanking sequence of the human monoamine oxidase (MAO) genes linked to human growth hormone which was used as a reporter gene. SH-SY5Y neuroblastoma cells and 1242 MG glioma cells were compared under basal conditions as well as after treatments with different drugs. Under basal conditions, the relative reporter activities of the different promoter fragments were similar for both cell lines. No changes in promoter activities, were observed when cells were treated with L-deprenyl, lithium chloride or raclopride. In contrast, increases (2-3-fold) in both reporter gene expression and enzyme activity were observed after ethanol treatment of cells transfected with MAO-B fragments. Gel retardation analysis showed that ethanol caused changes in transcription factor binding to the MAO-B core promoter in both the SH-SY5Y and 1242 MG cell lines in a cell-type specific fashion.</description><subject>Alcohol Drinking</subject><subject>Antimanic Agents - pharmacology</subject><subject>Antipsychotic Agents - pharmacology</subject><subject>Astrocytoma</subject><subject>Biological and medical sciences</subject><subject>Central Nervous System Depressants - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Electrophoresis</subject><subject>Ethanol - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Lithium Chloride - pharmacology</subject><subject>Monoamine Oxidase - genetics</subject><subject>Monoamine Oxidase Inhibitors - pharmacology</subject><subject>Neuroblastoma</subject><subject>Promoter Regions, Genetic - physiology</subject><subject>Psychotropic Drugs - pharmacology</subject><subject>Raclopride</subject><subject>Salicylamides - pharmacology</subject><subject>Selegiline - pharmacology</subject><subject>Transcription, Genetic - drug effects</subject><subject>Transcription, Genetic - physiology</subject><subject>Tumor Cells, Cultured - drug effects</subject><subject>Tumor Cells, Cultured - enzymology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0300-9564</issn><issn>1435-1463</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkEtLxDAURoMo4_jYuBeyEBdCNe9m3Kk4KihudF0y6a2NtMnYpD7-vRkcdOnqcvkOH_cehA4oOaWElGeXc0JZSRnTG2hKBZcFFYpvoinhhBQzqcQ22onxlRBCaaknaKI1VzNOpqh9CD6Y3nnA4dPVJgJ-gbykwfhoB7dMLnjsPG7H3nhsoetwl-mIzzMDJvXgE_5wqcXL-GXbYGxy74DrYXyJ2PgaQ2qND90e2mpMF2F_PXfR8_z66eq2uH-8ubu6uC8sFywVDVUcBAPdlDPeQG0WlgkBVC0skVarEpqak4Wo6xIApCFSW84aoaTURjPFd9HxT-9yCG8jxFT1Lq7ONh7CGKtS8_x7lvQfSKXOJinN4MkPaIcQ4wBNtRxcb4avipJq5b_685_hw3XruOih_kXXwnN-tM5NtKZrsmbr4i_GqZJEMP4NJYaNnA</recordid><startdate>19960101</startdate><enddate>19960101</enddate><creator>EKBLOM, J</creator><creator>ZHU, Q.-S</creator><creator>CHEN, K</creator><creator>SHIH, J. 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Neuroglia</topic><topic>Lithium Chloride - pharmacology</topic><topic>Monoamine Oxidase - genetics</topic><topic>Monoamine Oxidase Inhibitors - pharmacology</topic><topic>Neuroblastoma</topic><topic>Promoter Regions, Genetic - physiology</topic><topic>Psychotropic Drugs - pharmacology</topic><topic>Raclopride</topic><topic>Salicylamides - pharmacology</topic><topic>Selegiline - pharmacology</topic><topic>Transcription, Genetic - drug effects</topic><topic>Transcription, Genetic - physiology</topic><topic>Tumor Cells, Cultured - drug effects</topic><topic>Tumor Cells, Cultured - enzymology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>EKBLOM, J</creatorcontrib><creatorcontrib>ZHU, Q.-S</creatorcontrib><creatorcontrib>CHEN, K</creatorcontrib><creatorcontrib>SHIH, J. 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C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoamine oxidase gene transcription in human cell lines : treatment with psychoactive drugs and ethanol</atitle><jtitle>Journal of Neural Transmission</jtitle><addtitle>J Neural Transm (Vienna)</addtitle><date>1996-01-01</date><risdate>1996</risdate><volume>103</volume><issue>6</issue><spage>681</spage><epage>692</epage><pages>681-692</pages><issn>0300-9564</issn><eissn>1435-1463</eissn><coden>JNTMAH</coden><abstract>In the present study transcriptional activities has been measured with different fragments of the 5'-flanking sequence of the human monoamine oxidase (MAO) genes linked to human growth hormone which was used as a reporter gene. SH-SY5Y neuroblastoma cells and 1242 MG glioma cells were compared under basal conditions as well as after treatments with different drugs. Under basal conditions, the relative reporter activities of the different promoter fragments were similar for both cell lines. No changes in promoter activities, were observed when cells were treated with L-deprenyl, lithium chloride or raclopride. In contrast, increases (2-3-fold) in both reporter gene expression and enzyme activity were observed after ethanol treatment of cells transfected with MAO-B fragments. Gel retardation analysis showed that ethanol caused changes in transcription factor binding to the MAO-B core promoter in both the SH-SY5Y and 1242 MG cell lines in a cell-type specific fashion.</abstract><cop>Wien</cop><cop>New York, NY</cop><pub>Springer</pub><pmid>8836930</pmid><doi>10.1007/BF01271228</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0300-9564 |
| ispartof | Journal of Neural Transmission, 1996-01, Vol.103 (6), p.681-692 |
| issn | 0300-9564 1435-1463 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78383614 |
| source | Springer Online Journal Archives (Through 1996) |
| subjects | Alcohol Drinking Antimanic Agents - pharmacology Antipsychotic Agents - pharmacology Astrocytoma Biological and medical sciences Central Nervous System Depressants - pharmacology Dose-Response Relationship, Drug Electrophoresis Ethanol - pharmacology Fundamental and applied biological sciences. Psychology Humans Isolated neuron and nerve. Neuroglia Lithium Chloride - pharmacology Monoamine Oxidase - genetics Monoamine Oxidase Inhibitors - pharmacology Neuroblastoma Promoter Regions, Genetic - physiology Psychotropic Drugs - pharmacology Raclopride Salicylamides - pharmacology Selegiline - pharmacology Transcription, Genetic - drug effects Transcription, Genetic - physiology Tumor Cells, Cultured - drug effects Tumor Cells, Cultured - enzymology Vertebrates: nervous system and sense organs |
| title | Monoamine oxidase gene transcription in human cell lines : treatment with psychoactive drugs and ethanol |
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