Cell cycle control of BRCA2

Identifying the conditions and kinetics of the induction of BRCA2 gene expression may implicate roles for the function of the tumor suppressor gene. In this study, expression of BRCA2 mRNA is shown to be regulated by the cell cycle and associated with proliferation in normal and tumor-derived breast...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1996-10, Vol.56 (20), p.4590-4594
Main Authors: VAUGHN, J. P, CIRISANO, F. D, HUPER, G, BERCHUCK, A, FUTREAL, P. A, MARKS, J. R, IGLEHART, J. D
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
BRCA1 Protein - metabolism
BRCA2 Protein
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Cycle - drug effects
Cell Cycle - genetics
Cell Cycle - physiology
Cell cycle, cell proliferation
Cell Division
Cell physiology
Enzyme Inhibitors - pharmacology
Female
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Neoplastic
Histones - genetics
Histones - metabolism
Humans
Lovastatin - pharmacology
Molecular and cellular biology
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
RNA, Messenger - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Identifying the conditions and kinetics of the induction of BRCA2 gene expression may implicate roles for the function of the tumor suppressor gene. In this study, expression of BRCA2 mRNA is shown to be regulated by the cell cycle and associated with proliferation in normal and tumor-derived breast epithelial cells. Cells arrested in G(0) or early G1 contained low levels of BRCA2 mRNA. After release into a proliferating state, cells produced maximum levels of BRCA2 mRNA in late G1 and the S-phase. Similar cell cycle control of BRCA2 was observed in fractions of exponentially growing cells isolated by centrifugal elutriation. Expression of BRCA2 was shown to be independent of bulk DNA synthesis. In addition, the kinetics of BRCA2 mRNA up-regulation appeared to be similar to those of BRCA1, suggesting that the two genes could be commonly controlled. These results imply that these two tumor suppressor genes are utilized during growth and may have a protective role in cellular proliferation.
ISSN:0008-5472
1538-7445