Bovine papilloma virus-transformed cells contain multiple E2 proteins

Genetic evidence suggests that the bovine papilloma virus type 1 (BPV) E2 open reading frame may encode at least two gene products involved in the regulation of viral gene expression. One, which is probably the full-length product, trans-activates transcription via an enhancer in the viral regulator...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-08, Vol.85 (16), p.5864-5868
Main Authors: Hubbert, N.L, Schiller, J.T, Lowy, D.R, Androphy, E.J
Format: Article
Language:English
Subjects:
3T3 cells
ADN
Amino acids
Animals
Antibodies
BIOLOGIA
BIOLOGIE
BIOLOGY
BOVINE PAPILLOMAVIRUS
Cattle
Cell Transformation, Viral
Cellular immunity
CODE GENETIQUE
CODIGO GENETICO
DNA
DNA, Viral - metabolism
GENE
GENES
GENETIC CODE
Immunoprecipitation
IMMUNOPRECIPITATION TESTS
Messenger RNA
Open reading frames
Papillomaviridae - genetics
PAPILLOMAVIRUS BOVIN
PROTEINAS
PROTEINE
PROTEINS
REACCION DE INMUNOPRECIPITACION
REACTION D'IMMUNOPRECIPITATION
Transcription, Genetic
Viral Proteins - analysis
Viral Proteins - immunology
VIRUS DEL PAPILOMA BOVINO
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Genetic evidence suggests that the bovine papilloma virus type 1 (BPV) E2 open reading frame may encode at least two gene products involved in the regulation of viral gene expression. One, which is probably the full-length product, trans-activates transcription via an enhancer in the viral regulatory region. A second, containing sequences from the 3′ end of the open reading frame, inhibits the transactivating activity of the first product. We now report the identification and initial characterization of three E2-encoded proteins, with mobilities corresponding to 48, 31, and 28 kDa in cells transformed by the wild-type BPV. Pulse-chase experiments indicated that the 48-kDa protein had the longest half-life (40 min), but there was no indication that one species was the precursor of another. The 48-kDa species corresponds to the full-length trans-activating protein. The two smaller species contain only carboxyl-terminal determinants, and either or both could represent inhibitory E2 proteins. Subcellular fractionation localized all three E2 proteins to the nucleus. Consistent with the low rate of viral transcription in BPV-transformed cells, the 31-kDa presumptive repressor species was more abundant than the 48-kDa species.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.16.5864