Characterization of the sites of proteolytic activation of Newcastle disease virus membrane glycoprotein precursors

• Published in: The Journal of biological chemistry 1988-09, Vol.263 (25), p.12522-12531
• Main Authors: Gorman, J J, Nestorowicz, A, Mitchell, S J, Corino, G L, Selleck, P W
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; AUSTRALIA; AUSTRALIE; Base Sequence; Biological and medical sciences; Codon; Endopeptidases - metabolism; Fundamental and applied biological sciences. Psychology; GLICOPROTEINAS; GLYCOPROTEINE; GLYCOPROTEINS; Glycosylation; Hemagglutinins, Viral; HN Protein; Mass Spectrometry; Membrane Glycoproteins - metabolism; Membrane Proteins; Molecular Sequence Data; NEWCASTLE DISEASE VIRUS; Newcastle disease virus - analysis; NUCLEOTIDE; NUCLEOTIDES; NUCLEOTIDOS; Peptide Fragments - metabolism; Peptide Hydrolases - metabolism; Protein Biosynthesis; Protein Precursors - metabolism; Protein Processing, Post-Translational; Proteins; Serine Endopeptidases; Trypsin - metabolism; Viral Envelope Proteins - genetics; Viral Envelope Proteins - metabolism; Viral Fusion Proteins - metabolism; VIRUS DE LA ENFERMEDAD DE NEWCASTLE; VIRUS MALADIE DE NEWCASTLE
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)37786-X

The F1- and F2-polypeptide components of the fusion proteins and the hemagglutinin/neuraminidase proteins of the avirulent Queensland (V4) and virulent Australia-Victoria (AuV) strains of Newcastle disease virus have been isolated and subjected to extensive primary structural analysis including amino-terminal sequence analysis and fast atom bombardment-mass spectrometry mapping. Nucleotide sequence analysis was performed on the gene which encodes the V4 hemagglutinin/neuraminidase protein. Signal peptidase cleavage was found to have occurred at the Ser31-Leu32 peptide bond of the primary translation products of the fusion protein genes. Activation cleavage of the V4 fusion protein precursor generated a sequence of -Gly-Lys-Gln-Gly84 at the carboxyl terminus of the F2-polypeptide and an amino-terminal sequence of the F1-polypeptide commencing with 86Leu-Ile-Gly-. The V4 hemagglutinin/neuraminidase protein gene was found to encode a primary translation product 45 amino acids longer at the carboxyl terminus than obtainable from the corresponding gene of the AuV strain (McGinnes, L. W., and Morrison, T. G. (1986) Virus Res. 5, 343-356). However, post-translational proteolytic processing, exclusive to the primary translation product of the V4 hemagglutinin/neuraminidase protein gene, was found to have removed the last 42 residues of this carboxyl-terminal appendage.