Differential Regulation of HLA-DR Expression and Antigen Presentation in Toxoplasma gondii-Infected Melanoma Cells by Interleukin 6 and Interferon γ

CD4+ cytotoxic T lymphocytes (CTL) clones, YT-4 and YT-9, specific for Toxoplasma gondii (T. gondii)-infected melanoma SK-MEL 28 (P36), were generated from the peripheral blood lymphocytes (PBL) of a patient with chronic toxoplasmosis. These CTL clones were shown to secrete significant amounts of in...

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Published in:MICROBIOLOGY and IMMUNOLOGY 1996, Vol.40(6), pp.443-449
Main Authors: Yang, Tian-Hui, Aosai, Fumie, Norose, Kazumi, Ueda, Masakatsu, Yano, Akihiko
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Antigen Presentation - immunology
Biological and medical sciences
CD4-Positive T-Lymphocytes - cytology
CD4-Positive T-Lymphocytes - immunology
Cell interactions
Clone Cells
Cytokines - immunology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
HLA-DR Antigens - immunology
Humans
IFN-γ
IL-6
Immunobiology
Interferon-gamma - immunology
Interleukin-6 - immunology
Melanoma
T-Lymphocytes, Cytotoxic - cytology
T-Lymphocytes, Cytotoxic - immunology
Toxoplasma - immunology
Toxoplasma gondii
Toxoplasmosis - blood
Toxoplasmosis - immunology
Tumor Cells, Cultured
Up-Regulation
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Summary:CD4+ cytotoxic T lymphocytes (CTL) clones, YT-4 and YT-9, specific for Toxoplasma gondii (T. gondii)-infected melanoma SK-MEL 28 (P36), were generated from the peripheral blood lymphocytes (PBL) of a patient with chronic toxoplasmosis. These CTL clones were shown to secrete significant amounts of interleukin 6 (IL-6) and interferon γ (IFN-γ) upon antigen (Ag)-specific stimulation. Downregulation of human leukocyte antigen (HLA)-DR surface expression and HLA-DR mRNA levels in P36 cells were observed when P36 cells were infected with T. gondii. Such downregulated HLA-DR expressions of T. gondii-infected P36 cells were upregulated by treatment with both recombinant IL-6 (rIL-6) and recombinant IFN-γ (rIFN-γ). The antigen-presenting ability of T. gondii-infected P36 cells to T. gondii-infected cell-specific CTL was enhanced by rIFN-γ but not by rIL-6. The present study reveals the existence of differential regulation of HLA-DR expression and Ag presentation in T. gondii-infected melanoma cells by IL-6 and IFN-γ.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1996.tb01091.x