Poly(DL-lactide-co-glycolide) microspheres as carriers for peptide vaccines

• Published in: Vaccine 1996-06, Vol.14 (9), p.879-885
• Main Authors: Ertl, Hildegund C.J., Varga, Istvan, Xiang, Zhi Quan, Kaiser, Kimberly, Stephens, LaDonna, Otvos, Laszlo
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Applied microbiology; Biocompatible Materials - chemistry; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; Lactic Acid; Mice; Mice, Inbred C3H; Microbiology; Microspheres; Molecular Sequence Data; Peptide; Peptides - chemistry; Peptides - immunology; PLG microspheres; Polyglycolic Acid; Polylactic Acid-Polyglycolic Acid Copolymer; Polymers - chemistry; Rabies Vaccines - chemistry; Rabies Vaccines - immunology; vaccine; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Conjugate - chemistry; Vaccines, Conjugate - immunology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/0264-410X(96)00005-9

Peptides carrying an immunodominant T-helper cell epitope delineated from the rabies virus nucleoprotein either alone or in combination with a linear B-cell epitope from the same protein were incorporated into three different formulations of poly(DL-lactide-co-glycolide) (PLG) which were distinct in their composition, and consequently in their peptide release rates. In vitro peptides incorporated into any of the PLG formulations stimulated a peptide-specific T-cell line. Upon subcutaneous immunization of mice, the PLG formulation that showed the fastest peptide release rate induced the best immune response. This immune response was in magnitude comparable or even superior to that induced by peptide emulsified in complete Freund's adjuvant.