Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences

Flower pigmentation patterns were scored in 185 sense Chalcone synthase (Chs) transgenotes and 85 antisense Chs transgenotes; upon first flowering, 139 (75%) of sense transgenotes were found to be phenotypically altered, as were 70 (82%) of the antisense transgenotes. The observed patterns document...

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Bibliographic Details
Published in:Plant molecular biology 1996-08, Vol.31 (5), p.957-973
Main Authors: Jorgensen, R.A. (California Univ., Davis, CA (USA). Dept. of Environmental Horticulture), Cluster, P.D, English, J, Que, Q, Napoli, C.A
Format: Article
Language:English
Subjects:
Acyltransferases - genetics
CALCONA
CHALCONE
CHALCONES
CODE GENETIQUE
CODIGO GENETICO
Crosses, Genetic
DNA, Antisense
DNA, Bacterial
FENOTIPOS
FLEUR
FLORES
FLOWERS
Gene Dosage
Gene Expression Regulation, Plant
GENETIC CODE
NUCLEOTIDE SEQUENCE
PETUNIA
Petunia hybrida
PHENOTYPE
PHENOTYPES
PIGMENTACION
PIGMENTATION
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants, Genetically Modified - enzymology
Plants, Genetically Modified - genetics
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Suppression, Genetic
TRANSGENIC PLANTS
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Summary:Flower pigmentation patterns were scored in 185 sense Chalcone synthase (Chs) transgenotes and 85 antisense Chs transgenotes; upon first flowering, 139 (75%) of sense transgenotes were found to be phenotypically altered, as were 70 (82%) of the antisense transgenotes. The observed patterns document the range of phenotypic variations that occur, as well as confirm and extend the finding that sense Chs constructs produce several types of morphology-based flower pigmentation patterns that antisense Chs constructs do not. Long-term monitoring for epigenetic variations in one population of 44 sense Chs transgenotes showed that 43 (98%) were capable of producing a cosuppression phenotype. The primary determinant of sense-specific patterns of cosuppression of Chs was found to be the repetitiveness and organization pattern of the transgene, not 'position effects' by, or 'readthrough' from, flanking plant DNA sequences. The degree of cosuppression observed in progeny of transgenotes carrying multiple, dispersed copies as compared to that observed with a single copy of the transgene suggests that sense cosuppression of Chs is subject to a transgene dosage effect.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00040715