Outbreak of Multiply Resistant Enterobacteriaceae in an Intensive Care Unit: Epidemiology and Risk Factors for Acquisition

• Published in: Clinical infectious diseases 1996-03, Vol.22 (3), p.430-436
• Main Authors: Lucet, Jean-Christophe, Chevret, Sylvie, Decré, Dominique, Vanjak, Dominique, Macrez, Annick, Bédos, Jean-Pierre, Wolff, Michel, Regnier, Bernard
• Format: Article
• Language: English
• Subjects: Adult; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; beta-Lactam Resistance; Biological and medical sciences; Clinical Articles; Digestive tract; Disease Outbreaks; Drug Resistance, Multiple; Emergency and intensive care: techniques, logistics; Enterobacteriaceae; Enterobacteriaceae - isolation & purification; Enterobacteriaceae Infections - epidemiology; Epidemiology; Female; Genotypes; Hospital admissions; Humans; Infections; Intensive care medicine; Intensive care unit. Emergency transport systems. Emergency, hospital ward; Intensive Care Units; Internships; Klebsiella Infections - epidemiology; Klebsiella Infections - microbiology; Klebsiella pneumoniae; Klebsiella pneumoniae - isolation & purification; Male; Medical sciences; Microbial colonization; Predisposing factors; Prospective Studies; Risk Factors
• ISSN: 1058-4838; 1537-6591
• DOI: 10.1093/clinids/22.3.430

A prospective study was initiated in an intensive care unit (ICU) where extended-spectrum β-lactamase- producing enterobacteriaceae (ESBLPE) were endemic. From July 1990 to July 1991, patients hospitalized for ⩾5 days were screened for ESBLPE acquisition by means of weekly rectal sampling and clinical cultures. Baseline characteristics and various ICU procedures in 62 cases of ESBLPE were compared with those for 205 patients without ESBLPE, with use of Cox's model. Risk for acquiring ESBLPE (Klebsiella pneumoniae in most cases) increased during the ICU stay, from 4.2% in the first week to 24% in the fourth week. Baseline characteristics were not different between the two groups. Urinary catheterization (P = .04) and arterial catheterization (P = .03) were independent risk factors for acquiring ESBLPE and probably reflected frequency of health care manipulations. The first site of ESBLPE acquisition was the digestive tract in 58 of the 62 patients; 28 infections developed in 22 patients, and these followed or occurred simultaneously with rectal colonization in 18 of those 22. DNA macrorestriction analysis suggested that the same strain was responsible for most cases. In conclusion, ESBLPE acquisition depends on length of stay in the ICU and the use of invasive procedures. Colonization is a prerequisite for infection.