Novel method for purification of staphylococcal enterotoxin A

A novel single-step procedure for the purification of staphylococcal enterotoxin A (SEA), namely, dye ligand affinity chromatography with the triazine dye Red A, was developed. SEA purified by this method produced a single band when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophore...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1988-07, Vol.54 (7), p.1761-1765
Main Authors: Reynolds, D, Tranter, H.S, Sage, R, Hambleton, P
Format: Article
Language:English
Subjects:
Biological and medical sciences
CHROMATOGRAPHIE
Chromatography, Affinity - methods
CROMATOGRAFIA
Electrophoresis, Polyacrylamide Gel
ENTEROTOXINAS
ENTEROTOXINE
Enterotoxins - isolation & purification
EPURATION
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
Immunodiffusion
INVESTIGACION
Isoelectric Focusing
PURIFICACION
RECHERCHE
STAPHYLOCOCCUS AUREUS
Triazines
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Summary:A novel single-step procedure for the purification of staphylococcal enterotoxin A (SEA), namely, dye ligand affinity chromatography with the triazine dye Red A, was developed. SEA purified by this method produced a single band when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The yield from 5 liters of culture supernatant was 0.113 g, corresponding to an overall yield of 55%. In some instances, purification of SEA from culture supernatants by dye ligand affinity chromatography produced two enterotoxin peaks that could be eluted from the column with 300 and 500 mM phosphate buffer (pH 6.8). Enterotoxin from these peaks produced a single band when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but multiple bands were observed on isoelectric focusing gels. This method of purification represents a significant improvement in time, yields, and purity of enterotoxin over previously published purification methods
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.54.7.1761-1765.1988