Association of Biofilm Production of Coagulase-Negative Staphylococci with Expression of a Specific Polysaccharide Intercellular Adhesin

An association between adherent biofilm production on tissue culture plates and expression of a specific polysaccharide intercellular adhesin (PIA), which is functionally involved in cell clustering, was investigated for 179 Staphylococcus epidermidis isolates. Of the S. epidermidis strains, 50.8% w...

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Bibliographic Details
Published in:The Journal of infectious diseases 1996-10, Vol.174 (4), p.881-883
Main Authors: Mack, Dietrich, Haeder, Michael, Siemssen, Nicolaus, Laufs, Rainer
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - physiology
Antigens
Antiserum
Biofilms
Blood
Coagulase - analysis
Concise Comunication
Epidermal cells
Infections
Polysaccharides
Polysaccharides, Bacterial - physiology
Staphylococcus
Staphylococcus epidermidis
Staphylococcus epidermidis - physiology
Tissue culture techniques
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Summary:An association between adherent biofilm production on tissue culture plates and expression of a specific polysaccharide intercellular adhesin (PIA), which is functionally involved in cell clustering, was investigated for 179 Staphylococcus epidermidis isolates. Of the S. epidermidis strains, 50.8% were biofilm producers (A570 of >0.1). There was a significant positive association between biofilm production and PIA expression: 86.8% of biofilm-producing S. epidermidis strains produced PIA as detected with a specific coagglutination assay. In contrast, 88.6% of the biofilm-negative isolates did not express PIA (P < .001). A linear association between the amount of PIA produced as detected by inhibition ELISA and the amount ofbiofilm produced was established for 49 S. epidermidis strains, representing a continuum from biofilm-negative to strongly biofilm-producing (r = .81, P < .001). Apparently, PIA is important for biofilm accumulation in the majority of clinical S. epidermidis isolates.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/174.4.881