The B2 receptor on cultured human decidua cells: release of arachidonic acid by bradykinin

In vitro studies on cultured human decidual cells present evidence for the expression of the bradykinin B2 receptor. This protein was localized by immunocytochemical methods at the cellular plasma membrane and the mRNA was found in cellular extracts by RT-PCR. As a biological effect the release of14...

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Bibliographic Details
Published in:Immunopharmacology 1996-06, Vol.33 (1-3), p.164-166
Main Authors: Rehbock, Joachim, Chondromatidou, Agni, Buchinger, Peter, Hermann, Andrea
Format: Article
Language:English
Subjects:
Arachidonic Acid - secretion
Bradykinin
Bradykinin - analogs & derivatives
Bradykinin - pharmacology
Bradykinin Receptor Antagonists
Cells, Cultured
Decidua
Decidua - drug effects
Decidua - metabolism
Decidua - secretion
Female
Fetal membrane
Human
Humans
Immunohistochemistry
Labour
Lipopolysaccharides - pharmacology
Pregnancy
Receptor
Receptor, Bradykinin B2
Receptors, Bradykinin - genetics
Receptors, Bradykinin - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
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Summary:In vitro studies on cultured human decidual cells present evidence for the expression of the bradykinin B2 receptor. This protein was localized by immunocytochemical methods at the cellular plasma membrane and the mRNA was found in cellular extracts by RT-PCR. As a biological effect the release of14C-AA was observed. Cells that had been incubated for 24 h with14C-AA showed a bradykinin (BK)-induced release of radioactivity into the culture medium. This response was prevented by the specific bradykinin B2 receptor antagonist HOE 140. Pretreatment with LPS resulted in an increase of the spontaneous release of radioactivity. Adding BK, a further release of about 40–50% was to be shown. We conclude that BK can play a role in the case of bacterial infection of the fetal membranes AND may stimulate labour by an augmented production of prostaglandins.
ISSN:0162-3109
DOI:10.1016/0162-3109(96)00034-3