Exogenous estrogen acts differently on production of estrogen receptor in the preoptic area and the mediobasal hypothalamic nuclei in the newborn rat

Expression of the estrogen receptor (ER) in the preoptic area (POA) and the mediobasal hypothalamus (MBH) in newborn female rats was studied by immunohistochemistry (IHC) and in situ hybridization histochemistry (ISHH). The number of ER immunoreactive (ER-IR) cells decreased and expression of ER mRN...

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Bibliographic Details
Published in:Neuroscience research 1996-07, Vol.25 (3), p.247-254
Main Authors: Orikasa, Chitose, Mizuno, Kazuya, Sakuma, Yasuo, Hayashi, Shinji
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn - physiology
DNA, Complementary - genetics
Down-regulation
Estradiol - pharmacology
Estrogen receptor
Female
Hypothalamus, Middle - metabolism
Mediobasal hypothalamus
Neonatal estrogenization
Polymerase Chain Reaction
Preoptic area
Preoptic Area - metabolism
Rats
Rats, Sprague-Dawley
Receptors, Estrogen - drug effects
Receptors, Estrogen - genetics
RNA, Messenger - metabolism
Transcription, Genetic
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Summary:Expression of the estrogen receptor (ER) in the preoptic area (POA) and the mediobasal hypothalamus (MBH) in newborn female rats was studied by immunohistochemistry (IHC) and in situ hybridization histochemistry (ISHH). The number of ER immunoreactive (ER-IR) cells decreased and expression of ER mRNA was suppressed in the arcuate (ARH) and the ventromedial (VMH) hypothalamic nuclei by daily injections of estradiol benzoate (EB) for ten consecutive days. In contrast, in the POA, expression of ER mRNA was not suppressed by EB treatment, while the ER immunoreactivity and the number of ER-IR cells was decreased by EB treatment. Results of quantification of ER mRNA by reverse transcription-polymerase chain reaction correlated well with results from ISHH: that is, ER mRNA expression decreased in the MBH but not in the POA. Thus, estrogen affects ER gene expression differently in these two brain regions.
ISSN:0168-0102
1872-8111
DOI:10.1016/0168-0102(96)01050-4