Liver and intestinal fatty acid binding proteins in control and TGF beta 1 gene targeted deficient mice

• Published in: Molecular and cellular biochemistry 1996-06, Vol.159 (2), p.149-153
• Main Authors: Fontaine, R.N, Gossett, R.E, Schroeder, F, O'Toole, B.A, Doetschman, T, Kier, A.B
• Format: Article
• Language: English
• Subjects: Animals; binding proteins; Carrier Proteins - biosynthesis; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; fatty acids; Fatty Acids - metabolism; Gene Deletion; gene expression; Gene Targeting; genes; Inflammation - metabolism; Intestinal Mucosa - metabolism; intestines; liver; Liver - metabolism; Mice; Mice, Inbred C3H; Mice, SCID; Myelin P2 Protein - biosynthesis; Neoplasm Proteins; Nerve Tissue Proteins; Severe Combined Immunodeficiency - metabolism; site-directed mutagenesis; Syndrome; Transforming Growth Factor beta - genetics; Transforming Growth Factor beta - physiology; transforming growth factor deficient mice; transforming growth factors; tumor necrosis factors; Wasting Syndrome - metabolism
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/BF00420917

The effect of transforming growth factor beta-1 (TGFbeta1) expression on fatty acid binding proteins was examined in control and two strains of gene targeted TGFbeta1-deficient mice. Homozygous TGFbeta1-deficient 129 X CF- 1, expressing multifocal inflammatory syndrome, had 25% less liver fatty acid binding protein (L-FABP) when compared to control mice. The decrease in L-FABP expression was not due to multifocal inflammatory syndrome since homozygous TGFbeta1-deficient/immunodeficient C3H mice on a SCID background had 36% lower liver L-FABP than controls. This effect was developmentally related and specific to liver, but not the proximal intestine, where L-FABP is also expressed. Finally, the proximal intestine also expresses intestinal-FABP (I-FABP) which decreased 3-fold in the TGFbeta1-deficient/immunodeficient C3H mice only. Thus, TGFbeta1 appears to regulate the expression of L-FABP and I-FABP in the liver and the proximal intestine, respectively.