Characterization of Langat virus antigenic determinants defined by monoclonal antibodies to E, NS1 and preM and identification of a protective, non-neutralizing preM-specific monoclonal antibody

Hybridomas secreting monoclonal antibodies (MAb) to the thick-borne encephalitis (TBE) group virus, Langat virus (LGTV), were prepared. Of more than 200 MAb screened, 19 antibodies, which cross-reacted with the etiologic agent of Central European encephalitis, were selected for further characterizat...

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Bibliographic Details
Published in:Virus research 1996-08, Vol.43 (2), p.125-136
Main Authors: Iacono-Connors, Lauren C, Smith, Jonathan F, Ksiazek, Thomas G, Kelley, Cynthia L, Schmaljohn, Connie S
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
Antigens, Viral - immunology
Cell Line
Cross Reactions
ENCEFALITIS
ENCEPHALITE
ENCEPHALITIS
Encephalitis Viruses, Tick-Borne
ENFERMEDADES TRANSM POR GARRAPATAS
Epitopes
Female
FLAVIVIRUS
Flavivirus - immunology
GLICOPROTEINAS
GLYCOPROTEINE
GLYCOPROTEINS
HIBRIDOMA
HYBRIDOMAS
HYBRIDOME
Immunization, Passive
Langat virus
Macaca mulatta
MALADIE TRANSMISSIBLE PAR TIQUES
Mice
Mice, Inbred BALB C
Mice, Inbred ICR
MONOCLONAL ANTIBODIES
Tick-borne encephalitis
TICKBORNE DISEASES
Viral Envelope Proteins - immunology
Viral Nonstructural Proteins - immunology
VIROSE
VIROSES
VIROSIS
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Summary:Hybridomas secreting monoclonal antibodies (MAb) to the thick-borne encephalitis (TBE) group virus, Langat virus (LGTV), were prepared. Of more than 200 MAb screened, 19 antibodies, which cross-reacted with the etiologic agent of Central European encephalitis, were selected for further characterization. Of these MAb, 14 were specific for LGTV E glycoprotein, two for the NS1 protein, and three for preM protein. The two NS1-specific MAb and two of the E-specific MAb reacted with all six of the other TBE group viruses tested while the remainder of the E-specific MAb failed to recognize at least one of the viruses. None of the MAb neutralized LGTV in cell culture assays, but one of the preM-specific MAb protected weanling mice against a virulent LGTV challenge. Although protective antibodies to E and NS1 proteins of TBE viruses were reported, our data provided the first evidence for protection by a non-neutralizing antibody to the preM or M protein of an y of the tick-borne flaviviruses.
ISSN:0168-1702
1872-7492
DOI:10.1016/0168-1702(96)01325-1