Structural elucidation of an extracellular polysaccharide produced by Lactobacillus helveticus

An extracellular polysaccharide produced by a strain of Lactobacillus helveticus isolated from cheese milk has been investigated. Sugar and methylation analysis together with 1H and 13C NMR spectroscopy revealed that the polysaccharide is composed of hexasaccharide repeating units. The sequence of s...

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Bibliographic Details
Published in:Carbohydrate research 1996-09, Vol.291, p.155-164
Main Authors: Staaf, Mikael, Widmalm, Göran, Yang, Zhennai, Huttunen, Eine
Format: Article
Language:English
Subjects:
Carbohydrate Conformation
Carbohydrate Sequence
Exopolysaccharide
Lactobacillus - chemistry
Lactobacillus helveticus
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Monosaccharides - analysis
NMR
Oligosaccharides - chemistry
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - isolation & purification
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Summary:An extracellular polysaccharide produced by a strain of Lactobacillus helveticus isolated from cheese milk has been investigated. Sugar and methylation analysis together with 1H and 13C NMR spectroscopy revealed that the polysaccharide is composed of hexasaccharide repeating units. The sequence of sugar residues was determined by use of two-dimensional nuclear Overhauser effect spectroscopy and heteronuclear multiple-bond correlation experiments. The structure of the repeating unit of the exopolysaccharide from L. helveticus is as follows: ▪
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(96)00166-8